- NeuroMab™ Anti-F-Spondin/SPON1 Antibody, Clone N24875P (CBP11839) (Cat#: NRZP-0822-ZP4740)
- NeuroMab™ Anti-CD32b Antibody, Clone NR130P (Cat#: NRP-0422-P1803)
- NeuroMab™ Anti-Amyloid Beta 1-15 Antibody,Clone NR2660P (Cat#: NRP-0422-P867)
- NeuroMab™ Mouse Anti-SHANK3 Monoclonal Antibody (CBP929) (Cat#: NAB-0720-Z3477)
- NeuroMab™ Mouse Anti-LRP1 Monoclonal Antibody (CBP3363) (Cat#: NAB-0720-Z6479)
- NeuroMab™ Anti-Integrin αvβ8 BBB Shuttle Antibody,Clone NR2431P (Cat#: NRZP-1222-ZP1218)
- Mouse Anti-SCN5A Monoclonal Antibody (CBP708) (Cat#: NAB-0720-Z2720)
- NeuroMab™ Anti-Tau Antibody,Clone NR2944P (Cat#: NRP-0422-P1684)
- NeuroMab™ Anti-SEZ6 Antibody, Clone NR30P (Cat#: NRP-0422-P517)
- NeuroMab™ Anti-SEZ6 Antibody, Clone NR28P (Cat#: NRP-0422-P515)
- Mouse Microglia Cell Line BV-2, Immortalized (Cat#: NCL2110P153)
- Green Fluorescent Alpha-synuclein SH-SY5Y Cell Line (Cat#: NCL2110P209)
- Human Glial (Oligodendrocytic) Hybrid Cell Line (MO3.13) (Cat#: NCL-2108P34)
- iNeu™ Human Midbrain Dopaminergic Neurons (Cat#: NCL-21P6-003)
- Human Brain Vascular Pericytes (Cat#: NCL-21P6-015)
- Mouse Glioma Cell Line GL-261-Luc (Cat#: NCL-2108P06)
- Mouse Microglia from C57BL/6 (Cat#: NCL-21P6-082)
- Rat Microglia Cell Line HAPI, Immortalized (Cat#: NCL2110P015)
- Mouse Hippocampal Neuron Cell HT22 (Cat#: NCL2110P001)
- Mouse Retinal Ganglion Cells (Cat#: NCL2110P145)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- pAAV-syn-FLEX-jGCaMP8s-WPRE (Cat#: NTA-2106-P066)
- AAV2/9-hSyn-Flpo-EGFP-WPRE-pA (Cat#: NTA-2012-ZP149)
- AAV-EF1a-mCherry-flex-dtA (Cat#: NRZP-0622-ZP616)
- AAV-mDLX-CRE-tdTomato (Cat#: NRZP-0622-ZP721)
- rAAV-CAG-DIO-G-Flamp1 (Cat#: NRZP-0722-ZP719)
- pAAV-syn-jGCaMP8m-WPRE (Cat#: NTA-2106-P062)
- Dextran-FITC (Cat#: NTA-2011-ZP110)
- PRV-CAG-EGFP (Cat#: NTA-2011-ZP14)
- Dextran-CYanine5.5 (Cat#: NTA-2011-ZP118)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein, HIRMab-TNFR (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein, cTfRMAb-TNFR (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-ASA BBB Shuttle Protein, HIRMab-ASA (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, cTfRMAb-IDUA (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein, HIRMab-SGSH (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, HIRMab-GDNF (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-IDS BBB Shuttle Protein, HIRMab-IDS (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, cTfRMAb-GDNF (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein, 8D3-IL-1RA (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein, HIRMab-NAGLU (Cat#: NRZP-0423-ZP506)
pH Indicator Products
![pH Indicators](static/img/pH-Indicators-1.jpg)
Changes in cytosolic intracellular pH play a central role in many physiological processes such as muscle contraction, nerve-impulse propagation, and cell signaling. Unlike intracellular free Ca2+ concentrations, which can rapidly change by perhaps 100-fold, intracellular pH varies by only fractions of a pH unit, and such changes may occur quite slowly. Creative Biolabs now offers a range of novel indicators enabling you to track pH in the cytosol and in particular organelles.
pH Indicators at a Glance
- BCECF
BCECF is the most popular fluorescent pH sensor. Its pKa of 6.98 is ideal because all cells have intracellular pH near 7.0, with physiological changes of no more than a few tenths of a pH unit. At low pH, the dye is weakly fluorescent but becomes more fluorescent with increasing pH. The excitation spectrum of the dye undergoes a slight shift with pH change, while the wavelength of the emission maximum remains unchanged. BCECF has an excitation spectrum with a maximum at 507 nm and an isoexcitation point at 438 nm. Intracellular pH can therefore be estimated by measuring the ratio of the fluorescence intensities at 500 and 450 nm. Commercial BCECF indicators are available in membrane-impermeant free acid and cell-permeable AM ester forms.
- Flubida-2 pH Indicators
Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidin-chimera proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is non-fluorescent until the probe has entered the cells and is hydrolyzed by cellular esterases. Flubida-2 is membrane-permeable and can be delivered into cells via simple incubation.
- Carboxyfluorescein pH Indicators
With a pKa of 6.5, Carboxyfluorescein (FAM) can be used as a pH indicator or cellular tracer. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. FAM is also available in membrane permeable diacetate form. 5-(and-6)-Carboxy-2',7'-dichlorofluorescein is similar to FAM with a pKa of 4.8. It is useful for detecting pH in more acidic environment.
Tracking pH in vesicles
Tracking internalization of fluorescent dextran is a routine method for analyzing pH changes in cellular compartments. Dextran conjugates of pHrodo dyes provide the most complete solution by allowing discrimination of vesicles from early endosomes to lysosomes. pHrodo dyes are non-fluorescent at neutral pH and provide signal intensity modulation from pH 2 to pH 9 with a choice of fluorescent wavelengths. By conjugating pHrodo Red together with Rhodamine Green onto a 10,000 MW dextran, the dual-labeled probe also provides a reference signal for tracking.
How to Select the Optimal pH Indicator for your Experiments?
Fluorescein derivatives are available as single isomer and mixed isomers. The isomers have the same spectral properties. Single isomer may be preferable for certain coupling reactions but mixed isomers are suitable for most applications.
Membrane-Permeant pH Indicators | Membrane-Impermeant pH Indicators | ||
BCECF AM Ester | pKa 6.97 | BCECF, free acid | pKa 6.97 |
5-Carboxyfluorescein diacetate (single isomer) | pKa 6.5 | 5-Carboxyfluorescein (5-FAM, single isomer) | pKa 6.5 |
6-Carboxyfluorescein diacetate (single isomer) | 6-Carboxyfluorescein (6-FAM, single isomer) | ||
5-(and-6)-Carboxyfluorescein diacetate (mixed isomers) | 5-(and-6)-Carboxyfluorescein (5(6)-FAM, mixed isomers) | ||
5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester | Flubi-2 | ||
Flubida-2 | 5-Carboxy-2',7'-dichlorofluorescein | pKa 4.8 | |
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate | pKa 4.8 | 5-(and-6)-Carboxy-2',7'-dichlorofluorescein | |
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester | 5-Carboxy-2',7'-dichlorosulfonefluorescein | pKa 4 |
Cytoplasmic pH Indicators | Vesicle pH Indicators | |
Standard filter set | FITC; TRITC; Requires excitation ratio to quantify pH | FITC; TRITC; Requires excitation ratio to quantify pH |
Fluorophore | pHrodo Green; pHrodo Red; BCECF | pHrodo Green; pHrodo Red; BCECF |
Cell permeant or impermeant? | Permeant | Impermeant |
Usage notes | Load in cell media | Loaded by endocytosis, microinjection, patch pipette of pinocytic loading agent |
Platforms | Flow cytometer, microplate reader, imaging | Flow cytometer, microplate reader, imaging |
For any interested products, please feel free to contact us for more information.
Other Related Products
- Calcium Indicators
- Sodium Indicators
- Magnesium Indicators
- Zinc Indicators
- Membrane Potential Indicators