Product

Calcitonin Receptor Assay Cell Line

[CAT#: NCL2110P301]

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Product Overview

Description

Cell line information: HiTSeeker CALCR cell line
Official Full Name: Human Calcitonin Receptor
DNA Accesion Number: GenBank AY430048
Host Cell: HEK293 cell line
Resistance: G418 (Geneticin)
Format: Cryopreserved vials
Storage: Liquid Nitrogen

Features

The HiTector CALCR contains HEK293 cells stably expressing unlabeled human calcitonin receptor (CALCR). The Creative Biolabs CALCR cell line is designed to determine compounds or analyze their ability to modulate calcitonin receptors. When calcitonin binds to CALCR, it activates G protein, which in turn triggers a cellular response mediated by second messengers (calcium and cAMP). This cell line has been validated by measuring the increase in calcium and cAMP in the cytoplasm. The high reproducibility of this assay allows monitoring of the CALC receptor activation process in high-throughput screening.

Research Areas

GPCR

Assay

Calcitonin Receptor Assay

Assay Description

Creative Biolabs' calcitonin receptor assay allows compounds to be measured or analyzed for their ability to modulate CALCR. When the ligand binds to CALCR, it activates the G protein, which in turn triggers a cellular response mediated by calcium and cAMP. Our CALCR test has been validated to measure the changes in cytoplasmic cAMP and calcium concentrations after being activated by calcitonin. The high reproducibility of this assay allows monitoring of the CALCR activation process in high-throughput screening.

Assay Characteristics

Readout: Calcium Flux & cAMP Flux

Agonist: Calcitonin

EC50 Agonist: 8.00 x 10⁶-8 M for Calcium Assay & 5.12 x 10⁶-11 for cAMP flux

Type of Assay: Cell-based/Functional

Detection Method: Fluorimetry for Calcium and HTRF for cAMP

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.