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Creative Biolabs
Product

NeuroDiff™ Neuronal Differentiation Medium (Human)

[CAT#: NCC200541ZP]

A specially formulated medium optimized for the preferential differentiation of ESC-derived human neural progenitor cells to a neuronal lineage.

Applications:
Cell Differentiation; Stem Cell Culture
Cell Types:
Neural Stem Cell; Neural Progenitor Cell; Embryonic Stem Cell

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Product Overview

Description

Estem ™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of Estem ™ human neural progenitor cells into neuronal lineages. This medium has been extensively validated on Estem ™ human neural progenitor cells. Using Estem ™ Neuronal Differentiation Medium, a relatively pure neuronal cell population (> 90% bIII-tubulin positive) can be obtained. Very low levels of astrocytes were detected (<0.5% GFAP positive). Provide sufficient media to provide 100 independent reactions in a volume of 1 mL or 10 reactions in a volume of 10 mL.

Cell Types

Neural Stem Cell; Neural Progenitor Cell; Embryonic Stem Cell

Species Reactivity

Human

Applications

Cell Differentiation; Stem Cell Culture

Application Notes

A specially formulated medium optimized for the preferential differentiation of EStem human neural progenitor cells to a neuronal lineage.

Research Areas

Stem Cell Research
Properties

Components

• L-Glutamine (Cat. No. NCC200543ZP)
• Estem™ Human Neural Progenitor Cells Kit (Cat. No. NCC200523ZP)
• Estem™ Neural Expansion Medium (Cat. No. NCC200529ZP)
• Estem™ Neural Freezing Medium (Cat. No. NCC200544ZP)
• Human Neural Stem Cell Characterization Kit (Cat. No. NCC200545ZP)
• Poly-L-ornithine (NCC200546ZP)
• Laminin (Cat. No. NCC200547ZP)
• Penicillin/Streptomycin Solution (Cat. No. NCC200548ZP)
• Cell Detachment Solution (Cat. No. NCC200550ZP)
• Chamber slides
• Tissue culture-ware
• Phosphate-Buffered Saline (1X PBS) (Cat. No. NCC200549ZP)
• Hemocytometer
• Microscope

Form

Liquid

Serum Level

Serum-Free

Shipping

Dry ice

Storage

The media should be stored at -20 ° C until ready to use. After thawing, fresh L-glutamine should be added to a final concentration of 2 mM. The thawed medium should be stored at a temperature of 2-8 ° C and should have a validity of 1 month. Dispense into small portions to avoid repeated heating before each use.

Quality Control

Each lot of media is tested for the optimal differentiation of Human Neural Progenitor Cells to a neuronal lineage.
Sterility Testing: Negative

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Publications

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For Research Use Only. Not For Clinical Use.
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