FLIPR Calcium 3 Assay Kit
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Lot Number
Description
Choose from six formulas to complement the goals you are interested in:
FLIPR Calcium 6 Assay Kit (Cat: NEK-2101-ZP15)
FLIPR Calcium 6-QF Assay Kit (Cat: NEK-2101-ZP16)
FLIPR Calcium 5 Assay Kit (Cat: NEK-2101-ZP17)
FLIPR Calcium 4 Assay Kit (Cat: NEK-2101-ZP18)
FLIPR Calcium 3 Assay Kit (Cat: NEK-2101-ZP19)
The FLIPR calcium assay kit utilizes calcium-sensitive dyes that are absorbed into the cytoplasm of cells during incubation. After the ligand-receptor binds, it releases intracellular calcium and binds to the dye, thereby increasing the fluorescence intensity. All FLIPR calcium assay kits use extracellular masking technology to block background fluorescence and increase the signal window of the assay. The FLIPR Calcium 6-QF Analysis Kit is an exception, which can achieve analytical performance without the use of masking techniques, making it ideal for quenching sensitive applications such as multiplexing with luminescent indicators.
Comparison of fluorescence signal response to calcium flux during acetylcholine activation of muscarinic M1 receptors in CHO M1 cells. This illustrates the signal-to-noise ratio provided by various FLIPR calcium assay kits using quenching technology. Combining the novel fluorophore and the reliable quenching technology of the FLIPR calcium 4 analysis kit, the FLIPR calcium 5 analysis kit shows a higher signal-to-noise ratio than previous kits.
Fig.2 Comparison of FLIPR calcium 4 and 3 assay kits
In a measurement with a particularly small calcium response, the FLIPR calcium 4 measurement kit (orange) has a lower background compared to the FLIPR calcium 3 measurement kit (green), resulting in an increase in the ΔF/Fo value. Assay: The endogenous response to carbachol at 10,000 cells/well in HEK 293 cells in a 384-well plate before assaying on the FLIPR Tetra system.
Fig.3 FLIPR Calcium 4 and 3 Assay Kit vs Competitor
In many cases, the performance of FLIPR calcium 4 and calcium 3 analysis kits is quite good, but the performance is better than competitive kits, as shown by the Creative Biolabs customer in the data above. Assay: Undisclosed non-adherent cells were stimulated with protein activator without removing the medium, and the assay was performed on the FLIPR 3 instrument.
Fig.1 Comparison of FLIPR calcium 5, 4 and 3 assay kits
Comparison of fluorescence signal response to calcium flux during acetylcholine activation of muscarinic M1 receptors in CHO M1 cells. This illustrates the signal-to-noise ratio provided by various FLIPR calcium assay kits using quenching technology. Combining the novel fluorophore and the reliable quenching technology of the FLIPR calcium 4 analysis kit, the FLIPR calcium 5 analysis kit shows a higher signal-to-noise ratio than previous kits.
Fig.2 Comparison of FLIPR calcium 4 and 3 assay kits
In a measurement with a particularly small calcium response, the FLIPR calcium 4 measurement kit (orange) has a lower background compared to the FLIPR calcium 3 measurement kit (green), resulting in an increase in the ΔF/Fo value. Assay: The endogenous response to carbachol at 10,000 cells/well in HEK 293 cells in a 384-well plate before assaying on the FLIPR Tetra system.
Fig.3 FLIPR Calcium 4 and 3 Assay Kit vs Competitor
In many cases, the performance of FLIPR calcium 4 and calcium 3 analysis kits is quite good, but the performance is better than competitive kits, as shown by the Creative Biolabs customer in the data above. Assay: Undisclosed non-adherent cells were stimulated with protein activator without removing the medium, and the assay was performed on the FLIPR 3 instrument.
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