FLIPR Calcium Assay Kits
FLIPR® Calcium Assay Kit is a platform for measuring changes in intracellular calcium, which can be used for drug discovery and basic research.
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Key advantage
• Universal mixed reading protocol can speed up the measurement workflow and increase throughput
• Excellent signal-to-noise ratio helps confirm endogenous or transiently transfected receptor activity during assay development
• Pre-optimized and validated protocols for FLIPR®Tetra and FlexStation® systems ensure routine and unconventional cell lines and targets
Choose from six formulas to complement the goals you are interested in:
FLIPR Calcium 6 Assay Kit (Cat: NEK-2101-ZP15)
FLIPR Calcium 6-QF Assay Kit (Cat: NEK-2101-ZP16)
FLIPR Calcium 5 Assay Kit (Cat: NEK-2101-ZP17)
FLIPR Calcium 4 Assay Kit (Cat: NEK-2101-ZP18)
FLIPR Calcium 3 Assay Kit (Cat: NEK-2101-ZP19)
Components
FLIPR Calcium 6-QF Assay Kit (Cat: NEK-2101-ZP16)
FLIPR Calcium 5 Assay Kit (Cat: NEK-2101-ZP17)
FLIPR Calcium 4 Assay Kit (Cat: NEK-2101-ZP18)
FLIPR Calcium 3 Assay Kit (Cat: NEK-2101-ZP19)
The FLIPR calcium assay kit utilizes calcium-sensitive dyes that are absorbed into the cytoplasm of cells during incubation. After the ligand-receptor binds, it releases intracellular calcium and binds to the dye, thereby increasing the fluorescence intensity. All FLIPR calcium assay kits use extracellular masking technology to block background fluorescence and increase the signal window of the assay. The FLIPR Calcium 6-QF Analysis Kit is an exception, which can achieve analytical performance without the use of masking techniques, making it ideal for quenching sensitive applications such as multiplexing with luminescent indicators.
Comparison of fluorescence signal response to calcium flux during activation of muscarinic M1 receptor carbachol in CHO M1 cells. This illustrates the signal-to-noise ratio provided by the homogeneous detection in the culture medium using the FLIPR calcium 6 detection kit. FLIPR calcium 6 analysis kit combines a new fluorophore and mature quenching technology, showing the highest signal-to-noise ratio.
Fig.2 Quencher free signal window
The new fluorophore of the FLIPR Calcium 6 detection kit shows the highest signal-to-noise ratio when performing no-wash detection (the initial medium is removed, replaced with detection buffer, and the dye is loaded without washing). Obtain the same high quality performance with or without masking dye.
Fig.3 Antagonism data with FLIPR calcium 5 assay kit
The FLIPR calcium 5 analysis kit and the FLIPR Tetra system were used to evaluate the antagonistic effect of EC80 acetylcholine on calcium flux in CHO M1 cells, demonstrating a larger signal window and performance consistent with previously published data.
Fig.1 FLIPR calcium 5/6 analysis kit signal window
Comparison of fluorescence signal response to calcium flux during activation of muscarinic M1 receptor carbachol in CHO M1 cells. This illustrates the signal-to-noise ratio provided by the homogeneous detection in the culture medium using the FLIPR calcium 6 detection kit. FLIPR calcium 6 analysis kit combines a new fluorophore and mature quenching technology, showing the highest signal-to-noise ratio.
Fig.2 Quencher free signal window
The new fluorophore of the FLIPR Calcium 6 detection kit shows the highest signal-to-noise ratio when performing no-wash detection (the initial medium is removed, replaced with detection buffer, and the dye is loaded without washing). Obtain the same high quality performance with or without masking dye.
Fig.3 Antagonism data with FLIPR calcium 5 assay kit
The FLIPR calcium 5 analysis kit and the FLIPR Tetra system were used to evaluate the antagonistic effect of EC80 acetylcholine on calcium flux in CHO M1 cells, demonstrating a larger signal window and performance consistent with previously published data.
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