Product
Human 3D HUVEC Model Tissue (Blocks, Lysates, and Slides)
[CAT#: NCL-2103-P185]
- Species:
- Human
- Applications:
- In Vivo Like Assay
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Angiogenesis is a multi-step process in which new blood vessels develop from the original vascular system. Angiogenesis plays a key role in many physiological and pathological processes. Therefore, understanding the mechanism of angiogenesis will provide new ways to treat a wide range of pathologies. Angiogenesis is a complex process in which the following events are considered to play a key role: the proteolytic degradation of the extracellular matrix. The directional migration of endothelial cells. Endothelial cells proliferate. Deposit new extracellular matrix. The formation of tubules and the anastomosis of new blood vessels.
Our three-dimensional human HUVECs angiogenesis model is co-cultured with gfp-labeled human umbilical vein endothelial cells (HUVECs) and rfp-labeled Sertoli cells. The formation of GFP-positive capillary-like tubules can be monitored in real time under a fluorescence microscope throughout the entire experimental process.
The design of the 3D model is that test materials, namely compounds, conditioned media or tissue explants, can be added to the system at any time, ranging from the beginning of angiogenesis to late angiogenesis. Under the inverted fluorescence microscope, the effect on the formation of tubules (tubule length, number of branches, etc.) can be monitored throughout the process.
Our three-dimensional human HUVECs angiogenesis model is co-cultured with gfp-labeled human umbilical vein endothelial cells (HUVECs) and rfp-labeled Sertoli cells. The formation of GFP-positive capillary-like tubules can be monitored in real time under a fluorescence microscope throughout the entire experimental process.
The design of the 3D model is that test materials, namely compounds, conditioned media or tissue explants, can be added to the system at any time, ranging from the beginning of angiogenesis to late angiogenesis. Under the inverted fluorescence microscope, the effect on the formation of tubules (tubule length, number of branches, etc.) can be monitored throughout the process.
Applications
In Vivo Like Assay
Species
Human
Properties
Size
12 well
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Frozen (Dry Ice/Liquid Nitrogen)
Storage
Keep frozen in liquid nitrogen until plating
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
