Product

Human Endoneural Endothelial Cells (Thendec), Immortalized

[CAT#: NCL-2108P151]

Species:: Human

Applications:: Cell Culture

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Product Overview

Description

Immortalized human endoneurium endothelial cells (THEndEC) is a useful model for promoting receptor-mediated BNB permeability and/or kinetic studies in vitro, in addition to its sensitivity to drug poisoning studies, because it retains specialized transporters (Ie P-gp, γ-GT, LAT-1, CRT and MCT-1) and restricted barrier function cells similar to primary neuroendothelial endothelium. THEndEC has been characterized as showing specific binding to UEA-1 lectin and the ability to uptake Dil-Ac-LDL, indicating α-L-fucose expression and specialized scavenger receptor expression, respectively. Further research proved the use of THEndEC's transendothelial resistance (TEER) and permeability to sodium fluorescein and dextran, making this cell line also suitable for peripheral nerve permeability studies.

Applications

Cell Culture

Species

Human

Properties

Size

>1x10^6 Cells

Growth Pattern

Adherent

Morphology

spindle

Immortalization Method

Continuous passage and stable transfection with plasmids carrying SV40 large T antigen

Freeze Medium

90%FBS+10%DMSO

Tissue Source

Brain

Shipping

Dry ice.

Storage

Liquid Nitrogen

Research Use Only

For research use only

Quality Control

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.

Publications