Product
MagicCatch cAMP Fluorescent Assay Kit
[CAT#: NEK-2101-ZP21]
Cell signaling through G protein-coupled receptors (GPCR) can be assessed by monitoring the downstream effector calcium or cyclic AMP (cAMP).
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The high-affinity reagent of the cAMP fluorescence assay kit is optimized for sensitivity and accuracy for applications with low cAMP levels. A single washing step can remove unbound material before the development step, so the assay is very resistant to interference from colored or fluorescent test compounds. The proprietary Stoplight Red substrate is used to generate stable fluorescence readings, allowing measurement results to be read within as little as 10 minutes or up to 24 hours after the substrate is added.
Advantages:
High-affinity reagents are optimized for sensitivity at low cAMP levels to ensure results when other assays fail.
The robust format resists interference from colored or fluorescent compounds, thereby increasing the reliability of the results.
Fast signal generation (<10 minutes) and no termination step provide users with flexible reading time, compatible with automation and high-throughput screening.
The quantitative method can accurately determine cAMP in the sample.
Advantages:
High-affinity reagents are optimized for sensitivity at low cAMP levels to ensure results when other assays fail.
The robust format resists interference from colored or fluorescent compounds, thereby increasing the reliability of the results.
Fast signal generation (<10 minutes) and no termination step provide users with flexible reading time, compatible with automation and high-throughput screening.
The quantitative method can accurately determine cAMP in the sample.
Properites
Components
Explorer Kit (96-Wells)
Two (2) 96-well black well/ clear bottom plates
100 mL ELISA Assay Buffer
1 vial Rabbit Anti-cAMP Antibody
1 vial cAMP Calibrator
1 vial HRP-cAMP Conjugate
100mL, 10X Wash Solution Concentrate
50 mL Cell Lysis Buffer
1 vial 100X Substrate
100 mL Substrate Buffer
Bulk Kit (96-Wells)
Ten (10) 96-well black well/ clear bottom plates
950 mL ELISA Assay Buffer
2 vials Rabbit Anti-cAMP Antibody
2 vials cAMP Calibrator
2 vials HRP-cAMP Conjugate
950mL, 10X Wash Solution Concentrate
450 mL Cell Lysis Buffer
2 x 900 µL 100X Substrate
950 mL Substrate Buffer
Two (2) 96-well black well/ clear bottom plates
100 mL ELISA Assay Buffer
1 vial Rabbit Anti-cAMP Antibody
1 vial cAMP Calibrator
1 vial HRP-cAMP Conjugate
100mL, 10X Wash Solution Concentrate
50 mL Cell Lysis Buffer
1 vial 100X Substrate
100 mL Substrate Buffer
Bulk Kit (96-Wells)
Ten (10) 96-well black well/ clear bottom plates
950 mL ELISA Assay Buffer
2 vials Rabbit Anti-cAMP Antibody
2 vials cAMP Calibrator
2 vials HRP-cAMP Conjugate
950mL, 10X Wash Solution Concentrate
450 mL Cell Lysis Buffer
2 x 900 µL 100X Substrate
950 mL Substrate Buffer
Technology
Fig.1 MagicCatch cAMP measurement principle
The cAMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cAMP conjugate for the binding site on the anti-cAMP antibody. In the absence of cAMP, most HRP-cAMP conjugates bind to antibodies. Increasing the concentration of cAMP competitively reduces the amount of bound conjugate, thereby reducing the measured HRP activity.
The cAMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cAMP conjugate for the binding site on the anti-cAMP antibody. In the absence of cAMP, most HRP-cAMP conjugates bind to antibodies. Increasing the concentration of cAMP competitively reduces the amount of bound conjugate, thereby reducing the measured HRP activity.
Data
Fig.1 cAMP calibration curve
The calibration curve of the CAMP detection kit is generated on the LinearMax M5 multi-mode microplate reader. Data were obtained 24 hours after adding Stoplight Red substrate. The EC50 of the cAMP calibration curve is 5.1 nM.
Fig.2 Forskolin dose response in HEK293 cells
10,000 cells were stimulated in a volume of 20 μL for 15 minutes, then lysed and analyzed using the MagicCatch cAMP fluorescence analysis kit. The data was generated on the LinearMax M5 multi-mode microplate reader.
The calibration curve of the CAMP detection kit is generated on the LinearMax M5 multi-mode microplate reader. Data were obtained 24 hours after adding Stoplight Red substrate. The EC50 of the cAMP calibration curve is 5.1 nM.
Fig.2 Forskolin dose response in HEK293 cells
10,000 cells were stimulated in a volume of 20 μL for 15 minutes, then lysed and analyzed using the MagicCatch cAMP fluorescence analysis kit. The data was generated on the LinearMax M5 multi-mode microplate reader.
Product Pictures
FuncS
Fig.1 cAMP calibration curve
The calibration curve of the CAMP detection kit is generated on the LinearMax M5 multi-mode microplate reader. Data were obtained 24 hours after adding Stoplight Red substrate. The EC50 of the cAMP calibration curve is 5.1 nM.
FuncS
Fig.2 Forskolin dose response in HEK293 cells
10,000 cells were stimulated in a volume of 20 μL for 15 minutes, then lysed and analyzed using the MagicCatch cAMP fluorescence analysis kit. The data was generated on the LinearMax M5 multi-mode microplate reader.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
