NeuroMab™ Anti-6xHis BBB Shuttle Antibody, Clone N144/14

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-6xHis BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 NeuroMabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis (also known as Hexahistidine and Polyhistidine) by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Wide Species

Clonality

Monoclonal

Isotype

IgG1

Clone Number

N144/14

Applications

ELISA; DB; ICC; WB

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

6xHis

Official Name

6xHis

Full Name

6xHis

Product Pictures

Fig. 1 Immunofluorescence of COS cells expressing 6xHis/Myc-tagged human VDAC1. Green=N144/14, red=Rabbit anti-Myc, blue=Hoechst nuclear stain.

Fig. 2 Right: immunoblot against extracts of COS cells transiently transfected with 6xHis/Myc-tagged Olig2 or untagged Kv2.1 plasmid probed with N144/14 (left), 19E10 (middle) or K89/34 (right) TC supe.

Publications