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Creative Biolabs
Product

NeuroMab™ Anti-Glypican 3 Antibody, Clone NR21P

[CAT#: NRP-0422-P504]

A functional antibody raised against Human Glipican3.

Host Species:
Human
Species Reactivity:
Human
Applications:
FC; IHC; Cyt; ADCC; CDC; In Vitro; In Vivo

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Product Overview

Description

The human monoclonal antibody is specifically against human Glipicano-3 with high binding affinity.

Immunogen

A recombinant fusion protein, Glipicano-3, is created comprising the extracellular domain of the Glipicano-3 protein linked to non-Glipicano-3 polypeptide, a histidine brand.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Clone Number

NR21P

Applications

FC; IHC; Cyt; ADCC; CDC; In Vitro; In Vivo
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

Glypican 3

Official Name

GPC3

Full Name

glypican 3

Alternative Names

SGBS1; DGSX; Glypican-3; Intestinal protein OCI-5; GTR2-2; SGB; OCI-5; SDYS; MXR7; SGBS

Gene ID

2719(Human);14734(Mouse)

Uniprot ID

P51654(Human);Q8CFZ4(Mouse)
Product Pictures
FCM

Figure 1 shows the binding affinities of monoclonal antibodies 4A6, 11E7 and 16D10 to the CHC Hep-3B cell line.

Elimination is measured using an antibody-dependent cellular cytotoxicity assay ("ADCC").

FCM

Figure 2 shows that monoclonal antibodies 4A6, 11E7 and 16D10 specifically bind to the Hep-G2 cell line. Binding was measured using a FACS assay.

Elimination is measured using an antibody-dependent cellular cytotoxicity assay ("ADCC").

FCM

Figure 3 shows that monoclonal antibodies 4A6, 11E7 and 16D10 specifically bind to CHO cells stably transfected with a Glipicano-3 expression vector, but not to CHO progenitor cell lines.

Elimination is measured using an antibody-dependent cellular cytotoxicity assay ("ADCC").

IHC

Figure 4 shows the binding of monoclonal antibodies to liver cancer cells taken from patients.

Elimination is measured using an antibody-dependent cellular cytotoxicity assay ("ADCC").

ADCC

Figure 5 shows that monoclonal antibodies 4A6, 11E7, and 16D10 can mediate natural cytolytic T cell ("NK")-mediated depletion of Hep-3B cells.

Elimination is measured using an antibody-dependent cellular cytotoxicity assay ("ADCC").

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For Research Use Only. Not For Clinical Use.
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