Parkin Mitochondrial Recruitment Assay

In recent years, increasing evidence has shown that parkin promotes mitochondrial degradation through vesicle formation, which is closely related to the quality control of mitophagy. Therefore, the role of parkin in mitophagy and its association with neurodegenerative diseases have attracted widespread interest. As a reputable global service provider, Creative Biolabs specializes in providing comprehensive parkin mitochondrial recruitment assay to customers around the world to help you gain a deeper understanding of the mechanism of mitophagy.

Parkin Recruitment and Mitophagy

Functional mitochondria are linked to maintaining cellular survival. Furthermore, mitochondrial dysfunction has been implicated in various neurodegenerative diseases (ND) pathogenesis, such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and Huntington's disease (HD). Among the numerous mechanisms, two gene products mutated in PD, PINK1, and parkin, have attracted much attention. Parkin is a ubiquitin ligase E3 that binds ubiquitin to damaged mitochondrial proteins for degradation. PINK1 is a Ser/Thr kinase that accumulates only in damaged mitochondria where it activates parkin's E3 ubiquitin ligase activity. This multiple signaling mode ultimately results in damaged mitochondria being phagocytosed and degraded by lysosomes. Therefore, the assay of parkin mitochondrial recruitment is considered to be a promising predictive tool for exploring neurodegenerative pathobiology and identifying potential therapeutic approaches that can enhance mitophagy.

Fig.1 Model for the mechanism of parkin activation and recruitment by PINK1. (Nguyen, et al., 2016)

Parkin Mitochondrial Recruitment Assay

Recent research has shown that parkin has great potential to reveal the truth of mitophagy. Creative Biolabs has been focusing on mitophagy for decades and established a comprehensive technology platform. In our assay, the parkin mitochondrial recruitment assay is performed based on changes in the localization of two fluorescent proteins: the parkin fluorescent fusion polypeptide that transfers its localization to the outer mitochondrial membrane (OMM) and the other fluorescent fusion polypeptide (MTS-tGFP) that binds directly to the OMM. In addition to traditional assays, we also established a parkin mitochondrial recruitment assay on the cell line. This cellular model is a powerful tool that allows the identification of compounds that inhibit mitochondrial membrane potential loss through parkin recruitment.

Parkin is a critical component of the mitochondrial quality control system. Mitophagy mediated by parkin helps prevent the accumulation of toxic mitochondrial products, which leads to the loss of PD neurons and prevents damaged mitochondria from merging with healthy networks. Therefore, recent studies have focused on how PINK1/parkin acts on mitochondria to drive mitophagy. At Creative Biolabs, our platform is equipped with the latest technology and experienced scientists, we are confident in providing customers with reliable parkin mitochondrial recruitment assay services. Please contact us in time for more details.

Reference

1. Nguyen, T.N.; et al. Deciphering the molecular signals of PINK1/Parkin mitophagy. Trends in cell biology. 2016, 26(10): 733-744.