Rat Microglia Cell Line HAPI, Immortalized

Species:: Rat

Applications:: Cell Culture

Cell Types:: Microglia

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Product Overview

Features

Doubling time: 37h

Cell Types

Microglia

Applications

Cell Culture

Species

Rat

Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM high glucose medium (without sodium pyruvate) + 10% fetal bovine serum + 1% P/S

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Publications

Publications (0)

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Scientific Resources

[Neuroscience Biochemical Methods & Techniques]

Microglia Identification Methods

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[Flyer]

Cell Immortalization Service

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

An excellent addition to our lab

It’s highly responsive and shows consistent results in inflammatory response assays. The immortalized nature of these cells means we can rely on a steady supply without variability, which is crucial for our longitudinal studies.

Excellent

Impressed by their stability and ease of use

The cells are easy to culture and maintain, and their response to stimulants is very consistent. This has significantly reduced the variability in our experiments. They proliferate well and respond predictably to various experimental conditions. This cell line has streamlined our workflow considerably.

Excellent

Proven invaluable for our research on neuroinflammation

Their response to LPS stimulation is very robust, and we’ve had no issues with contamination or inconsistent results. Highly recommend this cell line for any lab studying microglial function.

Excellent

These cells are fantastic for drug screening purposes

They maintain their characteristics over multiple passages, allowing us to conduct large-scale experiments without worrying about cellular changes.

Excellent

Excellent for genetic manipulation studies

They tolerate transfections well and show stable gene expression over time. The HAPI cell line has become a staple in our lab for studying gene function in microglia.

Q&As

How do HAPI cells differ from primary microglia in experiments?

HAPI cells provide a more consistent and reproducible model compared to primary microglia, which can vary between isolations and donor animals. While primary microglia offer a closer representation of in vivo conditions, HAPI cells are easier to culture and manipulate genetically. They provide reliable results in studies of microglial function and signaling pathways.

Are HAPI cells suitable for drug screening experiments?

Yes, HAPI cells are well-suited for drug screening and studying the effects of various compounds on microglial function. Their stable phenotype allows for consistent results across experiments. Researchers often use them to assess the anti-inflammatory properties of new drugs and to explore potential treatments for neurodegenerative diseases.

Is it possible to use HAPI cells in co-culture systems with neurons or astrocytes?

Absolutely, HAPI cells can be effectively used in co-culture systems with neurons or astrocytes. This setup allows researchers to study cell-cell interactions and the role of microglia in the central nervous system. Co-culturing HAPI cells with other cell types provides a more comprehensive model for investigating neuroinflammation and neurodegenerative processes.