- NeuroMab™ Anti-EPHB2 Antibody,Clone NR1370P (Cat#: NRP-0422-P1220)
- NeuroMab™ Mouse Anti-LRP1 Monoclonal Antibody (CBP3363) (Cat#: NAB-0720-Z6479)
- NeuroMab™ Anti-TREM2 BBB Shuttle Antibody, Clone NR19 (Cat#: NRZP-1022-ZP4114)
- NeuroMab™ Anti-Tau Antibody,Clone NR2218P (Cat#: NRP-0422-P2275)
- NeuroMab™ Anti-TREM2 Antibody, Clone NR65P (Cat#: NRP-0422-P792)
- NeuroMab™ Anti-GARP Antibody,Clone NR3348P (Cat#: NRP-0422-P1639)
- NeuroMab™ Anti-FGFR1 Antibody,Clone NR3547P (Cat#: NRP-0422-P1244)
- Tau Monoclonal Antibody (AT120, HT7 and BT2 clone) (Cat#: NK-2106-P008)
- NeuroMab™ Anti-Alpha Synuclein Antibody,Clone NR3861P (Cat#: NRP-0422-P614)
- NeuroMab™ Anti-SEZ6 Antibody, Clone NR30P (Cat#: NRP-0422-P517)
- Human Microglia Cell Line HMC3, Immortalized (Cat#: NCL-2108P38)
- Rat Retinal Muller Cell Line, Immortalized (Cat#: NCL-21P6-192)
- Sf295 Human Glioblastoma Cells (Cat#: NCL-2108P180)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- iNeu™ Microglia (Cat#: NCL-7P018)
- Human Hippocampal Neuron Cells HPPNCs (Cat#: NCL2110P106)
- Mouse Glioma Cell Line GL-261-Luc (Cat#: NCL-2108P06)
- iNeu™ Human Schwann Cell (Cat#: NCL-2103-P63)
- Green Fluorescent Tau SH-SY5Y cell Line (Cat#: NCL2110P219)
- Mouse Glioma Cell Line GL261 (Cat#: NCL-2108P28)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- pAAV-syn-jGCaMP8f-WPRE (Cat#: NTA-2106-P061)
- pAAV-syn-jGCaMP8s-WPRE (Cat#: NTA-2106-P063)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- pAAV-EF1a-DIO-EGFP-WPRE (Cat#: NTA-2012AD-P285)
- AAV2/9-hSyn-Flpo-EGFP-WPRE-pA (Cat#: NTA-2012-ZP149)
- pAAV-syn-jGCaMP8m-WPRE (Cat#: NTA-2106-P062)
- pAAV-syn-FLEX-jGCaMP8s-WPRE (Cat#: NTA-2106-P066)
- AAV-mDLX-CRE-tdTomato (Cat#: NRZP-0622-ZP721)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- ABCA1 Antisense Oligonucleotide (AK311445) (Cat#: NV-2106-P27)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroPro™ Anti-IDS BBB Shuttle Protein, HIRMab-IDS (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein, HIRMab-PON1 (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, HIRMab-GDNF (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-EPO BBB Shuttle Protein, HIRMab-EPO (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, cTfRMAb-IDUA (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, HIRMab-IDUA (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein, cTfRMAb-TNFR (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein, HIRMab-SGSH (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, cTfRMAb-GDNF (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein, 8D3-IL-1RA (Cat#: NRZP-0423-ZP497)
Brain Mitochondria Assay
Mitochondria influence synaptic activity, calcium signaling, and reactive oxygen species (ROS) regulation, all of which are critical to neuronal communication and survival. Dysfunctional mitochondria have been implicated in numerous neurological disorders, including Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis (ALS).
Creative Biolabs offers a comprehensive Brain Mitochondria Assay service that unravels the mysteries of these organelles and their significance in neurological well-being.
Examples of Mitochondria Assays We Perform
Creative Biolabs' Brain Mitochondria Assay delves into multiple aspects of mitochondrial health, providing a holistic view of their functionality and integrity. Our assay covers the following key aspects:
Assays | Methodology and Measurement |
---|---|
Mitochondrial Membrane Potential (∆ψm) Assay | JC-1 dye is commonly used, and it changes color from green to red as the mitochondrial membrane potential increases, indicating healthy mitochondria. A decrease in red fluorescence suggests mitochondrial dysfunction. |
ATP Production Assays | Neurons require ATP for their function, and mitochondria are the primary source of ATP production. Various assays, such as luciferase-based assays, can measure ATP levels, reflecting mitochondrial activity and overall cellular energy status. |
Mitochondrial Respiration Assays | These assays measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of neurons, reflecting mitochondrial respiration and glycolytic activity, respectively. Seahorse XF analyzers are commonly used for real-time measurements of these parameters. |
Mitochondrial ROS (Reactive Oxygen Species) Assays | Mitochondria produce ROS as a byproduct of respiration. Excessive ROS can damage cellular components, including mitochondria. Dihydroethidium (DHE) and MitoSOX are commonly used fluorescent probes to measure mitochondrial ROS levels. |
Mitochondrial DNA (mtDNA) Copy Number Assays | This assay quantifies the relative abundance of mitochondrial DNA compared to nuclear DNA. Changes in mtDNA copy number can indicate mitochondrial dysfunction or changes in mitochondrial biogenesis. |
Mitochondrial Calcium Imaging Assays | Mitochondria play a role in calcium buffering in neurons. Fluorescent calcium indicators, such as Rhod-2 or Fura-2, can be used to monitor changes in mitochondrial calcium levels in response to neuronal activity. |
Mitochondrial Fusion and Fission Assays | These assays assess the dynamics of mitochondrial fusion and fission processes, which are crucial for maintaining mitochondrial health and function. |
Mitochondrial Enzyme Activity Assays | Enzymes involved in mitochondrial energy production, such as complexes of the electron transport chain, can be assayed to determine their activity levels. |
Mitochondrial Transport Assays: | Studying mitochondrial movement within neurons using live-cell imaging techniques can offer insights into mitochondrial dynamics. |
Notes: When selecting assays for brain studies, it's essential to choose methods that align with the specific research question and experimental model. Combining multiple assays can provide a comprehensive understanding of mitochondrial function and its impact on brain health and disease.
Our Advantages
Timely Delivery
We are committed to delivering results in a timely manner, allowing researchers to advance their projects efficiently.
Customization
Our experienced scientists can tailor the assay protocols and models to suit your specific requirements, ensuring the best fit for your project.
Expertise
Our team of highly skilled experts possesses extensive experience in the field of neuroscience research, ensuring the highest quality of service and reliable results.
Creative Biolabs assists researchers in exploring the mechanisms of how mitochondria affect the nervous system. Researchers can thus develop novel therapies to target neurodegenerative diseases through our comprehensive platform. Please contact us if you want to learn more about in vitro mitochondria-related assays.