Brain Mitochondria Assay

Mitochondria influence synaptic activity, calcium signaling, and reactive oxygen species (ROS) regulation, all of which are critical to neuronal communication and survival. Dysfunctional mitochondria have been implicated in numerous neurological disorders, including Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis (ALS).

Creative Biolabs offers a comprehensive Brain Mitochondria Assay service that unravels the mysteries of these organelles and their significance in neurological well-being.

Examples of Mitochondria Assays We Perform

Creative Biolabs' Brain Mitochondria Assay delves into multiple aspects of mitochondrial health, providing a holistic view of their functionality and integrity. Our assay covers the following key aspects:

Assays	Methodology and Measurement
Mitochondrial Membrane Potential (∆ψm) Assay	JC-1 dye is commonly used, and it changes color from green to red as the mitochondrial membrane potential increases, indicating healthy mitochondria. A decrease in red fluorescence suggests mitochondrial dysfunction.
ATP Production Assays	Neurons require ATP for their function, and mitochondria are the primary source of ATP production. Various assays, such as luciferase-based assays, can measure ATP levels, reflecting mitochondrial activity and overall cellular energy status.
Mitochondrial Respiration Assays	These assays measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of neurons, reflecting mitochondrial respiration and glycolytic activity, respectively. Seahorse XF analyzers are commonly used for real-time measurements of these parameters.
Mitochondrial ROS (Reactive Oxygen Species) Assays	Mitochondria produce ROS as a byproduct of respiration. Excessive ROS can damage cellular components, including mitochondria. Dihydroethidium (DHE) and MitoSOX are commonly used fluorescent probes to measure mitochondrial ROS levels.
Mitochondrial DNA (mtDNA) Copy Number Assays	This assay quantifies the relative abundance of mitochondrial DNA compared to nuclear DNA. Changes in mtDNA copy number can indicate mitochondrial dysfunction or changes in mitochondrial biogenesis.
Mitochondrial Calcium Imaging Assays	Mitochondria play a role in calcium buffering in neurons. Fluorescent calcium indicators, such as Rhod-2 or Fura-2, can be used to monitor changes in mitochondrial calcium levels in response to neuronal activity.
Mitochondrial Fusion and Fission Assays	These assays assess the dynamics of mitochondrial fusion and fission processes, which are crucial for maintaining mitochondrial health and function.
Mitochondrial Enzyme Activity Assays	Enzymes involved in mitochondrial energy production, such as complexes of the electron transport chain, can be assayed to determine their activity levels.
Mitochondrial Transport Assays:	Studying mitochondrial movement within neurons using live-cell imaging techniques can offer insights into mitochondrial dynamics.

Notes: When selecting assays for brain studies, it's essential to choose methods that align with the specific research question and experimental model. Combining multiple assays can provide a comprehensive understanding of mitochondrial function and its impact on brain health and disease.

Our Advantages

Timely Delivery

We are committed to delivering results in a timely manner, allowing researchers to advance their projects efficiently.

Customization

Our experienced scientists can tailor the assay protocols and models to suit your specific requirements, ensuring the best fit for your project.

Expertise

Our team of highly skilled experts possesses extensive experience in the field of neuroscience research, ensuring the highest quality of service and reliable results.

Creative Biolabs assists researchers in exploring the mechanisms of how mitochondria affect the nervous system. Researchers can thus develop novel therapies to target neurodegenerative diseases through our comprehensive platform. Please contact us if you want to learn more about in vitro mitochondria-related assays.

For Research Use Only. Not For Clinical Use.

Related Section

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Neuronal Mitochondrial Membrane Potential Assay

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ER/Autophagic Stress Assay

Nuclear Inclusions Assay

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