PRV-CAG-EGFP

[CAT#: NTA-2011-ZP14] Review(3) Q&As(3)

Retrograde, multisynaptic

Tracer Type:: Retrograde tracing virus

Applications:: Neural Tracing

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Product Overview

Description

Pseudorabies virus (PRV) has a double-stranded DNA genome with a genome of approximately 150 kb, which encodes capsid, outer skin, and envelope proteins. The reverse-transcribed anti-synaptic PRV is modified from the vaccine strain Bartha and can be used to analyze the connection structure of the brain center and peripheral neurons. After the virus infects nerve cells, it can replicate in the cell and express the target gene. The offspring virus is transported to the synapse, and then retrogrades the counter-synaptic label of the upstream neuron for further replication and transmission across the synapse.

After years of research, Creative Biolabs has established a stable production platform and can provide pseudorabies virus (PRV) services to promote PRV-based neuron tracking research.

Application field
●Multi-synaptic tracking from the periphery to the center of the brain or from higher to lower brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.

Tracer Type

Retrograde tracing virus

Virus Type

Pseudorabies Virus (PRV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Direction of Transport

Retrograde

Expression

Normal Expression

Properites

Fluorophore

EGFP

Storage

Please refer to the protocal.

Research Use Only

For research use only.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

We've been very pleased with the product's performance

This plasmid vector has surpassed our expectations. The EGFP fluorescence is bright and stable, making it ideal for live-cell imaging. PRV-CAG-EGFP has become a staple in our lab for tracking gene expression and studying cellular processes.

Excellent

The transfection efficiency is high

The PRV-CAG-EGFP vector has been invaluable for our in vivo experiments. The strong, ubiquitous expression driven by the CAG promoter allows for clear visualization of transfected cells. It's a reliable tool that has significantly advanced our research capabilities.

Excellent

This plasmid is a must-have for our studies

PRV-CAG-EGFP has been a reliable tool for our gene editing experiments. The high expression levels and stability of the EGFP signal have been crucial for our fluorescence microscopy studies. This plasmid has definitely enhanced the quality of our research.

Q&As

What is the recommended method for administering PRV-CAG-EGFP in animal models?

PRV-CAG-EGFP is typically administered via stereotactic injection directly into the targeted brain regions of animal models. This precise method ensures localized infection and optimal tracing of neural pathways. It’s crucial to follow detailed surgical procedures and use appropriate anesthesia to minimize distress and ensure the success of the experiment.

For how long can I expect to observe EGFP expression after infection with PRV-CAG-EGFP?

The expression of EGFP can typically be observed for several weeks post-infection. However, the duration may vary depending on factors like the site of injection, the animal model used, and the overall health of the organism. Monitoring should be conducted regularly to assess the fluorescence intensity and neuronal labeling over time.

Is PRV-CAG-EGFP suitable for retrograde tracing of neural circuits?

Yes, PRV-CAG-EGFP is commonly used for retrograde tracing, allowing researchers to map neural pathways by infecting neurons that project to the site of injection. This method provides insights into the source of neuronal inputs, helping to identify and characterize neural circuits and their functional roles within the nervous system.