VSV-eGFP

[CAT#: NTA-2011-ZP20] Review(5) Q&As(3)

Anterograde, multisynaptic

Tracer Type:: Anterograde tracing virus

Applications:: Neural Tracing

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Product Overview

Description

Vesicular stomatitis virus (VSV) is a non-segmented negative-strand RNA virus in the virus genus of the Rhabdoviridae family. The genome of VSV is approximately 11 kb-12 kb nucleotides, which encode nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and large polymerase protein (L). Improved VSV has been widely used as an anterograde tracer.
Glycoprotein (G) is an important component of VSV anterograde synapse. When G (VSV-ΔG) is removed, it will lose the ability to cross synapses, but it can still express foreign genes in high abundance. Therefore, VSV-ΔG can be used to quickly obtain a complete image of neuron projection distribution, and can also be used for anterograde single synapse tracking.

Creative Biolabs has established a recombinant VSV platform based on our experienced employees and advanced molecular biology technology, and can provide VSV services to promote VSV-based neuron tracking research.

Applications
●Multi-synaptic tracking from the periphery to the center of the brain or from lower to higher brain regions.
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.

Tracer Type

Anterograde tracing virus

Virus Type

Vesicular Stomatitis Virus (VSV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Direction of Transport

Anterograde

Expression

Normal Expression

Properites

Fluorophore

EGFP

Storage

Please refer to the protocal.

Research Use Only

For research use only.

References

1. Hastie E, Grdzelishvili VZ. Vesicular stomatitis virus as a flexible platform for oncolytic virotherapy against cancer. J Gen Virol. 2012 Dec;93(Pt 12):2529-45.
2. Bishnoi, S., Tiwari, R., Gupta, S., Byrareddy, S.N., Nayak, D. Oncotargeting by Vesicular Stomatitis Virus (VSV): Advances in cancer therapy. Viruses. 2018; 10(2): 90.
3. Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, Whelan SP, Sabatini B, Cepko CL. Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Delivered impressive results

The VSV-eGFP product was pivotal for our neural tracing studies. The fluorescence was highly visible and stable throughout our experiments, providing clear and reliable imaging of neural pathways. The ease of use and consistency in results make it a top choice for similar research.

Excellent

The product significantly enhanced the quality of our research data

We used VSV-eGFP for tracing neural circuits in our recent study. The GFP signal was robust and allowed for high-resolution imaging of neuronal connections. The product performed well and showed minimal background fluorescence, which was crucial for our analysis.

Excellent

Reliable and high quality

The VSV-eGFP worked excellently in our experiments. The fluorescent signal was bright and lasted throughout the study period. It facilitated precise tracing of neural networks, which significantly advanced our research. Highly recommend for anyone working on neural tracing.

Excellent

An invaluable tool

We found VSV-eGFP to be a reliable tool for our neural tracing applications. The GFP signal was strong and consistent, providing clear images of neuronal pathways. The product's quality was exceptional, and it met all our experimental needs.

Excellent

Our experience with VSV-eGFP has been very positive

Using VSV-eGFP was a great decision for our neural tracing work. The fluorescence was intense and stable, which allowed for detailed visualization of neural connections. The product was easy to handle and provided excellent results.

Q&As

How stable is the eGFP signal in VSV-eGFP for long-term neural tracing studies?

The eGFP signal in our VSV-eGFP is highly stable, maintaining robust fluorescence over extended periods. Depending on the application and experimental conditions, you can expect the signal to remain clear and consistent for several weeks. This stability ensures precise neural tracing, even in long-term studies, allowing for accurate tracking of neuronal pathways without significant signal degradation.

Is VSV-eGFP suitable for use in different model organisms, such as mice and rats?

Yes, VSV-eGFP is versatile and has been successfully used in various model organisms, including mice and rats. The product is optimized for neural tracing across species, providing consistent and high-quality fluorescent labeling. It is particularly effective in rodents for studies involving circuit mapping, allowing you to trace complex neural pathways with clarity in different models.

What kind of imaging system is best suited for visualizing the eGFP fluorescence in VSV-eGFP?

For optimal visualization of the eGFP fluorescence in VSV-eGFP, we recommend using a fluorescence microscope equipped with a filter set for GFP detection (excitation 488 nm, emission 509 nm). Confocal microscopy is ideal for high-resolution imaging, allowing for detailed examination of neural structures and pathways. Standard epifluorescence microscopes can also deliver good results, depending on your imaging needs.