Product
SmartTox™ Caspase-3/7 NucView 488 Assay Kit
[CAT#: NEK-2101-ZP08]
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The kit can detect the activity of caspase-3/7 using a fluorescence microplate reader or a fluorescence imaging system, so as to detect the apoptosis of a complete cell population. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. NucView 488 Caspase-3 substrate (prepared in DMSO or phosphate buffered saline (PBS) for SmartTox Caspase-3 / 7-D NucView 488 detection kit and SmartTox Caspase-3 / 7 NucView 488 detection kit, respectively) Detecting caspase-3/7 activity in intact cells does not interfere with caspase activity. The substrate consists of a fluorescent DNA dye coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the substrate penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate and releases a high-affinity DNA dye that migrates to the nucleus and stains the DNA with bright green fluorescence, excited at 500 nm, and emitted at 530 nm. NucView 488 staining can fix formaldehyde and is compatible with subsequent immunostaining. The assay kit includes optional masking reagents to reduce background fluorescence when necessary. The PBS preparation is recommended for cells sensitive to DMSO.
Properites
Components
Explorer Kit
(1) vial of NucView 488 Caspase-3 Substrate in PBS (1 mM, 200 µL)
(2) vials of masking reagent (1 vial/plate)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
Bulk Kit
(1) vial of NucView 488 Caspase-3 Substrate in PBS (1 mM, 1,000 µL)
(1) vial of masking reagent (1 vial/10 plates)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
(1) vial of NucView 488 Caspase-3 Substrate in PBS (1 mM, 200 µL)
(2) vials of masking reagent (1 vial/plate)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
Bulk Kit
(1) vial of NucView 488 Caspase-3 Substrate in PBS (1 mM, 1,000 µL)
(1) vial of masking reagent (1 vial/10 plates)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
Data
Fig.1 Representative data from LinearMax microplate reader
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
Product Pictures
FuncS
Fig.1 Representative data from LinearMax microplate reader
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
Publications
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For Research Use Only. Not For Clinical Use.
