Product

SmartTox™ Cell Integrity Kit

[CAT#: NEK-2101-ZP02]

The SmartTox™ Cell Integrity Kit enables the differentiation of live cells from dead cells via fluorescent labeling. This is useful for the rapid quantification of cell viability.

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Product Overview

Description

The SmartTox Cell Integrity Kit is an optimized set of reagents to simplify the identification of live and dead cells. It can be used to measure the effects of different treatments on cell viability and to evaluate the toxic effects mediated by multiple mechanisms, including apoptosis and necrosis. The kit is designed for many types of cells, including adherent and non-adherent cells. The simple workflow and reagent performance of this scheme make it suitable for high-throughput screening.

Features
Higher signal allows shorter exposure time for faster results
Designed for use with multiple cell types
Simplified image acquisition and analysis on a single system

Properites

Components

Explorer Kit
(2) Reagent vials (one red, one green)*
* Sufficient for (2) 96- or 384-well plates

Bulk Kit
(2) Reagent vials (one red, one green)**
** Sufficient for (10) 96- or 384-well plates

SmartTox Live Green Dye
(1) Reagent vial*
* Sufficient for (2) 96- or 384-well plates

SmartTox Dead Green Dye
(1) Reagent vial*
* Sufficient for (2) 96- or 384-well plates

SmartTox Live Dye
(1) Reagent vial*
* Sufficient for (2) 96- or 384-well plates

Technology

Assay Kit Mechanism
The SmartTox Cell Integrity Kit uses two nuclear dyes to enable users to detect changes in the permeability of the outer cell membrane due to cell damage or cell death caused by necrosis, apoptosis or other mechanisms. Evaluate cell viability by counting the number or percentage of live cells and damaged cells. The assay can be used to study the effects of different treatments on cell viability, evaluate the toxic effects of pharmaceutical compounds or other chemicals, study necrosis and apoptosis, and many other applications.

Data

Fig.1 Image of untreated and compound-treated HeLa cells
(A) Untreated cells (left image) are mostly alive with red fluorescent nuclei. At the intermediate concentration of the compound (central part), there is a mixture of live and dead cells. At high compound concentrations (right panel), most cells die and the nuclei are marked in red and green. Acquire images on the LinearMax® MiniMax™ 300 imaging cytometer. (B) Use the classification function in SoftMax®Pro Software to identify cells as live cells (red mask) or dead cells (blue mask).

Fig.2 IC 50 curve of cytotoxic compounds
Treat HeLa cells with anisomycin (red circle) or staurosporine (blue square). Use the 4-parameter curve fitting in ProMax software to draw the results. Anisomycin has an IC50 of 3.3 μM and staurosporine has an IC50 of 0.53 μM; both values are consistent with the published values.

Product Pictures

FuncS

Fig.1 Image of untreated and compound-treated HeLa cells

A) Untreated cells (left image) are mostly alive with red fluorescent nuclei. At the intermediate concentration of the compound (central part), there is a mixture of live and dead cells. At high compound concentrations (right panel), most cells die and the nuclei are marked in red and green. Acquire images on the LinearMax® MiniMax™ 300 imaging cytometer. (B) Use the classification function in SoftMax®Pro Software to identify cells as live cells (red mask) or dead cells (blue mask).

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