SmartTox™ Cell Viability Assay Kits
SmartTox™ Cell Viability Assay Kit is a series of fluorescence-based reagents used to evaluate cell viability, cell proliferation and various apoptotic events using mammalian cells.
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Features
The best data quality of the microplate reader
Simplified similar experiment protocol
Pre-configured SoftMax Pro software protocol
The collection kits include:
SmartTox™ Live/Dead Assay Kit (Cat: NEK-2101-ZP04)
SmartTox™ Live Cell Assay Kit (Cat: NEK-2101-ZP06)
SmartTox™ Caspase-3/7 R110 Assay Kit (Cat: NEK-2101-ZP07)
SmartTox™ Caspase-3/7 NucView 488 Assay Kit (Cat: NEK-2101-ZP08)
SmartTox™ Glutathione Assay Kit (Cat: NEK-2101-ZP09)
Components
SmartTox™ Live Cell Assay Kit (Cat: NEK-2101-ZP06)
SmartTox™ Caspase-3/7 R110 Assay Kit (Cat: NEK-2101-ZP07)
SmartTox™ Caspase-3/7 NucView 488 Assay Kit (Cat: NEK-2101-ZP08)
SmartTox™ Glutathione Assay Kit (Cat: NEK-2101-ZP09)
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
Fig.2 IC 50 curve of cytotoxic compounds
Treat HeLa cells with anisomycin (red circle) or staurosporine (blue square). Use the 4-parameter curve fitting in ProMax software to draw the results. Anisomycin has an IC50 of 3.3 μM and staurosporine has an IC50 of 0.53 μM; both values are consistent with the published values.
Fig.1 Representative data from LinearMax microplate reader
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
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