Product
SmartTox™ Glutathione Assay Kit
[CAT#: NEK-2101-ZP09]
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The kit can use a fluorescent microplate reader to detect the content of glutathione in mammalian cells, thereby detecting early apoptosis events. Decreased cellular glutathione (GSH) is an important antioxidant in mammalian cells. Due to GSH efflux, it occurs in the early stages of mitochondrial-related apoptosis. This kit uses monochlorodiphenyldiamine (MCB), a cell-permeating dye with high affinity for GSH, to detect cellular GSH levels in apoptotic events. The unreacted dye hardly fluoresces. After the dye reacts with GSH, it is catalyzed by endogenous glutathione S-transferase (GST) enzyme, and the dye emits blue fluorescence, which is excited at 394 nm and emits at 490 nm. The intensity of the fluorescent signal generated by the measurement reflects the amount of GSH present in the cell.
Properites
Components
Explorer Kit
(1) vial of MCB (10 mM, 100 µL)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
Bulk Kit - PN
(1) vial of MCB (10 mM, 500 µL)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
(1) vial of MCB (10 mM, 100 µL)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
Bulk Kit - PN
(1) vial of MCB (10 mM, 500 µL)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
Data
Fig.1 Representative data from LinearMax microplate reader
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
Product Pictures
FuncS
Fig.1 Representative data from LinearMax microplate reader
Use data collected from HeLa cells. (A) Live/Dead: Cell viability measured by green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Apoptosis measured by caspase 3/7 activity; (C) Valley Glutathione: Apoptosis measured by glutathione content; D&E: Caspase-3/7 NucView488. The SmartTox Caspase-3 / 7 NucView 488 detection kit is also suitable for fluorescence imaging, because the nuclei of intact apoptotic cells are labeled according to their caspase activity. (D) Assess caspase 3/7 activity on a microplate reader; (E) Assess caspase 3/7 using fluorescence imaging.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
