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Creative Biolabs
Product

Stable HEK293T Expressing Mouse DMT1 Cell Line

[CAT#: NCL-2105-P317-AM]

These cells can be used for disease modeling, drug screening and toxicology testing.

Species:
Mouse

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Product Overview

Description

The mouse DMT1 cell line that stably expresses HEK293T overexpresses the divalent metal transporter 1 (DMT1) introduced into HEK293T cells by DMT1 cDNA transfection. This cell line at Creative Biolabs can be used for cell-based screening of small molecule inhibitors of iron uptake due to metal-sensitive calcein fluorescence.

Species

Mouse
Properties

Growth Pattern

Adherent

Morphology

Polygonal

Seeding Density

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Freeze Medium

90% FBS+10% DMSO

Tissue Source

Renal

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Negative

Sterility Testing

The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen on dry ice

Storage

Liquid nitrogen immediately upon delivery

Research Use Only

For research use only

Quality Control

1) Puromycin resistance at 2 µg/ml, 2) Western blot to confirm the presence of DMT1
Publications

Publications (0)

gift-card

For Research Use Only. Not For Clinical Use.
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