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Creative Biolabs
Product

NeuroMab™ Anti-CD123 Antibody, Clone NR09P

[CAT#: NRP-0422-P492]

A functional antibody raised against Human, Cynomolgus Monkey CD123.

Host Species:
Mouse
Species Reactivity:
Human; Cynomolgus Monkey
Applications:
FC; Cyt; ADCC; In Vitro; In Vivo

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Product Overview

Description

The antibody recognized domain A of IL-3Ra. The antibody is a therapeutic agent and a diagnostic agent for myelocytic malignant tumors

Species Reactivity

Human; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Mouse

Clone Number

NR09P

Applications

FC; Cyt; ADCC; In Vitro; In Vivo
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

CD123

Official Name

CD123

Full Name

interleukin-3 receptor

Alternative Names

IL3RA
Product Pictures
FCM

Figure 1 Flow cytometric analysis of cells expressing IL-3RalGM-CSFRa chimeric protein using anti-IL-3Ra marker antibody.

Antibodies were incubated with HepG2 cells at 37°C. Antibody bound to the cell surface was detected using a PE-conjugated anti-Fc antibody, but showed a FITC signal, representing both surface-exposed and internalized antibodies.

FCM

Figure 2 Flow cytometry analysis of cells expressing IL-3RalGM-CSFRa chimeric protein using anti-IL-3Ra marker antibody.

Antibodies were incubated with HepG2 cells at 37°C. Antibody bound to the cell surface was detected using a PE-conjugated anti-Fc antibody, but showed a FITC signal, representing both surface-exposed and internalized antibodies.

Block

Figure 3 Cell growth assay examining the blocking activity of IL-3 signaling.

The ordinate represents the percentage of cell growth inhibition (%), and the abscissa represents various names of anti-IL-3Ra antibodies.

FuncS

Figure 4 Anti-tumor effects of various human antibodies in tumor-bearing models.

The ordinate represents the number of MOLM13 cells, and the abscissa represents various names of anti-IL-3Ru antibodies.

ADCC

Figure 5 ADCC assay of IL3Ra expressing cell lines using anti-IL-3Ra antibody.

The ordinate represents the number of MOLM13 cells, and the abscissa represents various names of anti-IL-3Ru antibodies.

FCM

Figure 6 Flow cytometric analysis of cells expressing cynomolgus IL-3Ru.

The ordinate represents the number of MOLM13 cells, and the abscissa represents various names of anti-IL-3Ru antibodies.

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