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Creative Biolabs
Product

NeuroMab™ Anti-Glipican3 BBB Shuttle Antibody, Clone NR19P

[CAT#: NRZP-1022-ZP2422]

Host Species:
Human
Species Reactivity:
Human
Applications:
FC; IHC; Cyt; ADCC; CDC; In Vitro; In Vivo

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Certificate of Analysis Lookup
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Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Immunogen

A recombinant fusion protein, Glipicano-3, is created comprising the extracellular domain of the Glipicano-3 protein linked to non-Glipicano-3 polypeptide, a histidine brand.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Clone Number

NR19P

Applications

FC; IHC; Cyt; ADCC; CDC; In Vitro; In Vivo
Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only
Target

Target

GPC3

Official Name

GPC3

Full Name

glypican 3

Alternative Names

SGBS1; DGSX; Glypican-3; Intestinal protein OCI-5; GTR2-2; SGB; OCI-5; SDYS; MXR7; SGBS
Product Pictures
FCM

Fig.1 shows the binding affinity of monoclonal antibodies 4A6, 11E7 and 16D10 for the CHC Hep-3B cell line.

Binding was measured using a Fluorescence Activated Cell Classification ("FACS") assay.

FCM

Fig.2 shows that monoclonal antibodies 4A6, 11E7, and 16D10 specifically bind to the Hep-G2 cell lines. Binding was measured using an FACS assay.

Binding was measured using a Fluorescence Activated Cell Classification ("FACS") assay.

FCM

Fig.3 shows that monoclonal antibodies 4A6, 11E7, and 16D10 specifically bind to CHO cells stably transfected with a Glipicano-3 expression vector, but not to the progenitor CHO cell line.

Binding was measured using an FACS assay.

IHC

Fig.4 shows that monoclonal antibody binds to liver cancer cells taken from patients.

Binding to the antibody was revealed in an immunohistochemical assay.

ADCC

Fig.5 shows that monoclonal antibodies 4A6, 11E7, and 16D10, can mediate the elimination of Hep-3B cells mediated by natural cytolytic T cells ("NK").

Elimination was measured with an antibody-dependent cell cytotoxicity assay ("ADCC").

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