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Creative Biolabs
Product

NeuroMab™ Anti-Glipican3 BBB Shuttle Antibody, Clone NR21P

[CAT#: NRZP-1022-ZP2424]

Host Species:
Human
Species Reactivity:
Human
Applications:
FC; IHC; Cyt; ADCC; CDC; In Vitro; In Vivo

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Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Immunogen

A recombinant fusion protein, Glipicano-3, is created comprising the extracellular domain of the Glipicano-3 protein linked to non-Glipicano-3 polypeptide, a histidine brand.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Clone Number

NR21P

Applications

FC; IHC; Cyt; ADCC; CDC; In Vitro; In Vivo
Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only
Target

Target

GPC3

Official Name

GPC3

Full Name

glypican 3

Alternative Names

SGBS1; DGSX; Glypican-3; Intestinal protein OCI-5; GTR2-2; SGB; OCI-5; SDYS; MXR7; SGBS
Product Pictures
FCM

Fig.1 shows the binding affinity of monoclonal antibodies 4A6, 11E7 and 16D10 for the CHC Hep-3B cell line.

Elimination was measured with an antibody-dependent cell cytotoxicity assay ("ADCC").

FCM

Fig.2 shows that monoclonal antibodies 4A6, 11E7, and 16D10 specifically bind to the Hep-G2 cell lines. Binding was measured using an FACS assay.

Elimination was measured with an antibody-dependent cell cytotoxicity assay ("ADCC").

FCM

Fig.3 shows that monoclonal antibodies 4A6, 11E7, and 16D10 specifically bind to CHO cells stably transfected with a Glipicano-3 expression vector, but not to the progenitor CHO cell line.

Elimination was measured with an antibody-dependent cell cytotoxicity assay ("ADCC").

IHC

Fig.4 shows that monoclonal antibody binds to liver cancer cells taken from patients.

Elimination was measured with an antibody-dependent cell cytotoxicity assay ("ADCC").

ADCC

Fig.5 shows that monoclonal antibodies 4A6, 11E7, and 16D10, can mediate the elimination of Hep-3B cells mediated by natural cytolytic T cells ("NK").

Elimination was measured with an antibody-dependent cell cytotoxicity assay ("ADCC").

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