Viral vector tools for calcium imaging.
Creative Biolabs provides a comprehensive list of neural AAV viral tracing tools for calcium imaging.
Step1: Choose the appropriate calcium ion indicator
Calcium ion indicators are divided into chemiluminescent indicators and GECI indicators. Among them, GECI indicators GCaMP6f and GCaMP6s are widely used in in vivo calcium imaging research.
Step2: Transport the calcium ion indicator to the target site
The chemiluminescent indicator is usually pierced by an electrode or entered into the target site by means of acetoxymethyl and dextrose, while the GECI indicator is mainly introduced to the target site by means of viral infection.
Step3: Collect signals and record the results
Calcium imaging technology is usually used in conjunction with microscopy to record changes in calcium ions