NeuroMab™ Anti-Pan-Neurofascin (external) BBB Shuttle Antibody, Clone A12/18

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-Pan-Neurofascin BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin by:
-binding to NF-186-tranfected COS cells
-recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM
-immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry
-loss of immunostaining against NF186-shRNA knockdown samples
Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186)
Mouse: 98% identity (1074/1086 amino acids identical)
Human: 79% identity (955/1195 amino acids identical)

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Human; Rat

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

A12/18

Applications

WB; IHC; ICC

Research Areas

CAMs

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

Pan-Neurofascin

Official Name

Pan-Neurofascin

Full Name

Pan-Neurofascin (extracellular)

Alternative Names

NFASC; NF; NRCAML; neurofascin; NEDCPMD

Product Pictures

Fig. 1 Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.