NeuroMab™ Anti-TRIP8b (exon 1b) BBB Shuttle Antibody, Clone N212A/34

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-PEX5L BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Fusion protein amino acids 1-205 (exon 1b as MSDSEMDGRTHIPSLLNALLSRNRVMQMSYL, exon 2 and constant region) of mouse TRIP8b (also known as TPR-containing Rab8b-interacting protein, Peroxisome biogenesis factor 5-like, Peroxin-5- or PEX5-related/like protein, Pex5Rp, Pex5l, Pex5r, Pex2 and Pxr2, accession number Q8C437-3)
Rat:>90% identity (150/163 amino acids identical), 100% identity with 1b-specific sequence
Human:>90% identity (163/174 amino acids identical), 100% identity with 1b-specific sequence

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Rat; Mouse; Human

Clonality

Monoclonal

Isotype

IgG1

Clone Number

N212A/34

Applications

WB; IHC; ICC

Application Notes

Specific for exon 1b (does not cross-react with TRIP8b isoforms containing exons 1a, 2 or 4)

Research Areas

Ion channels

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

PEX5L

Official Name

PEX5L

Full Name

TRIP8b (exon 1b)

Alternative Names

PEX5R; PXR2; TRIP8b

Uniprot ID

Q8C437-3

Product Pictures

Fig. 1 immunoblot against crude membranes from whole rat (RBM) and mouse (MBM) brain and from human cerebellum (HBM(Cb)], cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N212A/34 (left) or N52A/42 (right) TC supe.

Fig. 2 Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212A/34 (left) or N86/8 (right) TC supe.

Fig. 3 Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212A/34 (left) and N52A/42 (right) TC supe.

Publications