Product
Trilineage Differentiation qPCR Array Kit for Human Pluripotent Stem Cell
[CAT#: NCC200652ZP]
Used to characterize the differentiation of hPSC three lines into endoderm, mesoderm and ectoderm germ layer
- Applications:
- Cell Culture
- Cell Types:
- iPSC; Embryonic Stem Cell; Neural Lineage Cell
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
Human pluripotent stem cell (hPSC) Trilineage differentiation quantitative polymerase chain reaction (qPCR) array is designed to characterize hPSC and its Trilineage differentiation ability. hPSC, including embryonic stem (ES) and induced pluripotent stem (iPS) cells, has the ability to self-renew and can differentiate into three germ layers: ectoderm, mesoderm and endoderm. The qPCR array provides gene expression profiles of undifferentiated ES and iPS cells and their three-line derivatives after directed or spontaneous differentiation in vitro, thereby verifying the ability of the cell line to differentiate into three germ layers. Genes are selected based on their differential expression in ES and iPS cells compared to hPSC-derived ectoderm, mesoderm and endoderm lineage cells.
qPCR is used to determine changes in steady-state mRNA levels of gene expression in multiple samples, which are usually normalized to the relative expression of internal control genes. Gene-specific primers can amplify target sequences in a cDNA library reverse-transcribed from mRNA, and, like TaqMan® technology, hybridized sequence-specific probes can provide fluorescence from the 5'exonuclease activity of Taq DNA polymerase signal. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, thereby quantifying the amount of mRNA in the original cell lysate.
The qPCR array contains validated primers and probes for the detection of 90 genes whose expression is related to undifferentiated hPSC or its derivatives at the early stage of differentiation, and six endogenous (housekeeping) control genes. Provide TBP (TATA Box Binding Protein) qPCR control template as a positive control for synthetic DNA.
qPCR is used to determine changes in steady-state mRNA levels of gene expression in multiple samples, which are usually normalized to the relative expression of internal control genes. Gene-specific primers can amplify target sequences in a cDNA library reverse-transcribed from mRNA, and, like TaqMan® technology, hybridized sequence-specific probes can provide fluorescence from the 5'exonuclease activity of Taq DNA polymerase signal. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, thereby quantifying the amount of mRNA in the original cell lysate.
The qPCR array contains validated primers and probes for the detection of 90 genes whose expression is related to undifferentiated hPSC or its derivatives at the early stage of differentiation, and six endogenous (housekeeping) control genes. Provide TBP (TATA Box Binding Protein) qPCR control template as a positive control for synthetic DNA.
Cell Types
iPSC; Embryonic Stem Cell; Neural Lineage Cell
Species Reactivity
Human
Applications
Cell Culture
Research Areas
Stem Cell Research
Properties
Components
hPSC Trilineage qPCR Array, 1 x 384-well plate
TBP qPCR Control Template, 1 vial
TBP qPCR Control Template, 1 vial
Shelf Life
Stable until expiry date (EXP) on label.
Research Use Only
For research use only
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
