Brain Neuron Cultures Recording Assay

Creative Biolabs is the world-leading CRO for in vitro electrophysiological assays dedicated to neurodegenerative diseases drug discovery. We provide exceptional expertise in the design, performance, and analysis of complex electrophysiological/pharmacological protocols. With a large bandwidth and short turnarounds, Creative Biolabs accelerates your neurodegenerative diseases drug discovery programs until the IND claim.

Description

Brain neuron cultures constitute an easy approach to investigating the effect of ion channel activators or inhibitors on membrane properties (ion channels). This type of assay offers mid-throughput capabilities and small series of compound profiling.

We perform recordings in neurons cultures and brain tissue from various rodent and human tissue:

• Human iPSC/ hESC- derived neurons
• Rodent brain neuron primary cultures
• Human brain neuron primary cultures
• Rodent acute brain slices
• Human acute brain slices
• Organotypic slices

We provide different brain regions with neuronal cultures:

• Hippocampal
• Cortical
• Striatal
• Cerebellar
• …

Manual Patch Clamp Technology

Manual patch-clamp electrophysiology allows for high-resolution recordings from a single neuron. Detailed physiological and pharmacological inquiries can be addressed with this technique.

The patch clamp technique is a versatile electrophysiological tool for understanding the activities of channels or receptors and a great number of studies have been conducted by applying this technique to brain neurons. We are able to physiologically characterize your cells / evaluate your compound’s efficacy on a single neuron down to single channel thanks to the different recording configurations (whole-cell, perforated-patch, cell-attached, outside-out).

Automated Patch Clamp Technology

We assessed the pharmacological manipulation of ion channel activators or inhibitors in primary neurons and brain tissue using patch-clamp electrophysiology. Our neurodegenerative ion channel portfolio includes ion channels relevant to diseases of the central and peripheral nervous system, covering more than 120 targets to guide early screening and selective analysis. When required, our scientists can design customized panels to meet a client’s needs.

Ion Channel Families:

• Acid-sensing ion channels (ASIC1a)
• Chloride (CFTR and CLC-2)
• Ligand-gated (NMDA (NR1/ NR2A, NRI/NR2B, NR1/NR2C and NR1/NR2D) and nAChR (α7 and α3 β4))
• Potassium, calcium-activated (SK2, SK3 and IK)
• Potassium, inward-rectifier (Kir6.2/SUR1)
• Potassium, voltage-gated (Kv1.1, Kv1.2, Kv1.3, Kv1.5, Kv2.1, Kv3.4 and KCNQ2/3)
• Sodium, voltage-gated (Nav1.1, Nav1.2, Nav1.3 and Nav1.6)
• Transient receptor potential (TRPC4)

Sample Data

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Reference

1. Wellbourne-Wood, J.; Chatton, J.Y. From cultured rodent neurons to human brain tissue: Model systems for pharmacological and translational neuroscience. ACS chemical neuroscience. 2018, 9(8): 1975-1985.