Mouse Microglia N9

[CAT#: NCL2110P073] Review(5) Q&As(3)

Species:: Mouse

Applications:: Cell Culture

Cell Types:: Microglia

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Product Overview

Description

Mouse microglia cells N9 were isolated from CD-1 mice and cultured adherently.

Features

Doubling time: 32h

Cell Types

Microglia

Applications

Cell Culture

Species

Mouse

Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM high glucose medium + 10% FBS + 1% P/S

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Excellent product

The N9 cells are easy to culture and maintain. They have shown great viability and robustness in our various experimental conditions.

Excellent

High purity and low variability

The N9 cells have shown excellent performance in our assays. Their consistent growth rates and predictable behavior have made our research more efficient and productive.

Excellent

Using Mouse Microglia N9 cells has been a smooth experience

We've had a positive experience with the N9 cells. They are easy to handle. N9 cells have become our go-to for microglial research. Their reproducibility and responsiveness to various experimental conditions have significantly enhanced the quality of our data.

Excellent

These microglial cells have a stable phenotype and are easy to maintain

The N9 microglial cells exhibit excellent viability and are straightforward to culture. This has made our long-term studies on microglial aging much more manageable and productive. Their reliable performance and ease of use have accelerated our experimental timelines significantly.

Excellent

Excellent for biochemical assays

We use N9 microglia to study the neurotoxic effects of various chemicals. Their robustness and reproducibility have made them a go-to model in our lab, providing consistent and reliable data.

Q&As

Are the mouse microglia N9 cells genetically engineered or modified in any way?

No, the mouse microglia N9 cells are not genetically engineered or modified. They are a stable cell line derived from mouse brain tissue and retain the characteristics of primary microglial cells.

How do I assess the activation state of cells in my experiments?

The activation state of N9 cells can be assessed using several methods. Common techniques include measuring the expression of activation markers such as CD11b, Iba1, and MHC class II using flow cytometry or immunocytochemistry. Additionally, you can quantify the production of pro-inflammatory cytokines like TNF-α, IL-1β, and IL-6 using ELISA or qPCR. Monitoring changes in cell morphology and phagocytic activity can also provide insights into their activation state.

Can I use the mouse microglia N9 cells for drug screening assays in my research project?

Yes, the mouse microglia N9 cells are suitable for drug screening assays in research projects. These cells offer a reliable and reproducible model for testing the effects of various compounds on microglial cell function.