Product
Human Blood Brain Barrier Model
[CAT#: NCL-2103-P187]
- Species:
- Human
- Applications:
- In Vivo Like Assay
- Cell Types:
- BBB Model
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The BBB model mimic the transport properties of the blood-brain barrier (BBB) due to the formation of tight junctions, high expression of specific vectors, or cell viability. We established a three-dimensional in vitro blood-brain barrier model by culturing brain endothelial cells and stratified pericytes and astrocytes. This model improves the polarization of endothelial cells and improves the formation of tight junctions. It is important to provide better development of contact barriers between endothelial cells. It is important to prevent the dilution of secreted neurotrophic factors. The development of these conditions can truly mimic the in vitro model of BBB.
Features:
The model is customized.
Models come with the media.
The BBB model can be used for transport and permeation studies from ions to macromolecules.
Features:
The model is customized.
Models come with the media.
The BBB model can be used for transport and permeation studies from ions to macromolecules.
Cell Types
BBB Model
Applications
In Vivo Like Assay
Species
Human
Properties
Size
6 well, 12 well, 24 well
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Frozen (Dry Ice/Liquid Nitrogen)
Storage
Keep frozen in liquid nitrogen until plating
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
