Human Blood Brain Barrier Model

Species:: Human

Applications:: In Vivo Like Assay

Cell Types:: BBB-related Cells

Cell Subtypes:: 3D Model

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Product Overview

Description

The BBB model mimic the transport properties of the blood-brain barrier (BBB) due to the formation of tight junctions, high expression of specific vectors, or cell viability. We established a three-dimensional in vitro blood-brain barrier model by culturing brain endothelial cells and stratified pericytes and astrocytes. This model improves the polarization of endothelial cells and improves the formation of tight junctions. It is important to provide better development of contact barriers between endothelial cells. It is important to prevent the dilution of secreted neurotrophic factors. The development of these conditions can truly mimic the in vitro model of BBB.

Features:
The model is customized.
Models come with the media.
The BBB model can be used for transport and permeation studies from ions to macromolecules.

Cell Types

BBB-related Cells

Cell Subtypes

3D Model

Applications

In Vivo Like Assay

Species

Human

Properties

Size

6 well, 12 well, 24 well

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen (Dry Ice/Liquid Nitrogen)

Storage

Keep frozen in liquid nitrogen until plating

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Donor Information

Donor Health

Normal

Publications

Publications (0)

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Scientific Resources

[Brochure]

Featured Products for Cellular Neuroscience Research

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[Flyer]

Engineering Therapeutics to Cross BBB

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[Hot Topics]

Blood-brain Barrier Permeability: Key to Neurological Health & Disease

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

An invaluable tool

It has streamlined our drug screening process, providing reliable data on compound permeability. The realistic simulation of the blood-brain barrier ensures that our findings are relevant and translatable to clinical settings. The setup is straightforward, and the support team is responsive and helpful.

Excellent

Become an essential part of our workflow

It accurately mimics the physiological conditions of the human brain, allowing us to test potential therapeutic agents with high precision. The model's durability and ease of use have saved us time and resources. Highly recommended for any research involving the central nervous system.

Excellent

The model is durable and easy to use

The in vivo-like conditions it provides have enabled us to conduct more precise and relevant experiments. The model is robust, and the results are consistent. This model is a must-have for any lab focused on brain research.

Excellent

I highly recommend it to other researchers

Its realistic simulation of the blood-brain barrier has provided us with valuable data on drug permeability and neurotoxicity. The model is robust and user-friendly, making it an essential tool for our research.

Excellent

Enabled us to conduct more precise and relevant experiments

The model's ability to mimic the physiological conditions of the human brain has improved the accuracy of our drug screening assays. The setup is straightforward, and the results are reproducible. This model has greatly enhanced our research efficiency.

Q&As

What are the key features of the SHT290 that make it suitable for studying drug delivery across the BBB?

The SHT290 model features high transendothelial electrical resistance (TEER) values, indicating tight junction integrity, crucial for BBB studies. It also provides reproducible results, consistent barrier properties, and compatibility with various assays, including permeability, toxicity, and transport studies. These attributes make it ideal for evaluating drug candidates' ability to penetrate the BBB effectively.

How do I culture the SHT290 model in my lab, and what are the maintenance requirements?

Culturing the SHT290 model involves specific protocols to ensure the integrity of the BBB. Begin by seeding the cells on a compatible insert, ensuring optimal cell density. Regularly monitor TEER values to assess barrier integrity. Maintenance requires changing media every 2-3 days and maintaining the culture under standard conditions of 37°C and 5% CO₂, ensuring a healthy and functional model.

How does the SHT290 model compare to other in vitro BBB models available on the market?

The SHT290 model is distinguished by its high reproducibility, physiological relevance, and tight junction integrity. Unlike some models that may use non-human cells or lack key cellular interactions, the SHT290 employs human-derived cells, offering a more accurate representation of the human BBB. Its robust performance in permeability and transport studies makes it a preferred choice for many researchers.