NeuroMab™ Anti-CD133 Antibody, Clone 47-10

Figure 1 shows a line graph illustrating the results of an ELISA specificity test for mAbs.

Figure 2 shows Western blot Figures of cell lysates from U87 cells (CD133 negative cells; lane 1 in each blot) and HT-29 cells (CD133 positive cells; lane 2 in each blot).

Panels A and B show Western blots stained with different subclones of mAb 47. Panels C and D show Western blots stained with different subclones of mAb 133 clone. The left lane of each blot shows molecular weight (MW) standards. The unlabeled arrows at the right of lane 2 in each panel point at a band for unglycosylated CD133 having a MW of 97 kDa. The arrows labelled "Glyc. CD133" point at an approximate location of a band for glycosylated CD133 having a MW of ˜133 kDa.

Figure 3 shows Western blot Figures of cell lysates from cell lines HT29, A375, SKMEL5, SKMEL28, and U87 (as marked, from left to right, the first left lane in each blot contains MW standards).

Panels A (decreased brightness) and B (increased brightness) show western blots using mAb 47-10. Panels C (decreased brightness) and D (increased brightness) show western blots using mAb 133-3.

Figure 4 shows Figures showing the results of immunofluorescence testing of mAb 47-10 with H29 cells (panel A) and A375 cells (panel B).

Figure 10 shows a scatter plot illustrating the quantification of CD133+CD44v6+ colocalized cells in slides prepared from Sum149-PT xenograft tumors

Figure 11 shows a scatter plot illustrating the quantification of CD133+ cells in slides prepared from Sum149-PT xenograft tumors

Figure 5 shows Figures illustrating the results of immunofluorescence testing of mAbs 133-3 (panel A) and 47-10 (panel B) with A375 xenografted cells. Cell pelleted FFPE samples were stained with mAb at 10 μg/ml for CD133 followed by anti-rabbit-AF546 antibody; nuclei were stained with DAPI.

Figure 6 shows Western blot Figures illustrating the results of a comparative test of mAbs 47-10 and 133-3 with a commercially available anti-CD133 mAb.

Figure 7 shows an Figure organized as a matrix depicting immunofluorescent staining of FFPE samples representing cells of cell lines with varying levels of CD133 mRNA expression

Figure 8 shows Figures depicting CD133 staining of selected tumor cores from Multitumor TMA (MTU951, Biomax) stained with CD133 (mAb 133-3, mAb 47-10, 293C3, or AC133) - as indicated.

Figure 9 shows Figures depicting CD133 staining results of selected tumor cores of melanoma TMA (ME1004c, Biomax) stained with mAbs133-3 and 47-10, as indicated in the graph.