DRG Neurons Peripheral Neurotoxicity Assay

Assessment of the potential neurotoxicity of candidate compounds is one of the most important part in drug development. Creative Biolabs is a leading neuroscience company that specializes in providing high-quality CRO services and products to support neurological diseases drug discovery and development. We have developed a cutting-edge assay for evaluating the neurotoxicity of drugs on dorsal root ganglion (DRG) neurons.

Culture and Characterization of DRG Neurons

Dorsal root ganglia (DRG) are clusters of sensory neurons located along the spinal cord. These neurons are responsible for transmitting sensory information from the periphery to the central nervous system (CNS). Therefore, studying DRG neurons is important for understanding the mechanisms of sensory perception and pain.

At Creative Biolabs, we provide high-quality DRG neuron cultures that ensure the maintenance of neuronal viability and functionality. We have extensive experience in isolating and characterizing DRG neurons from various species, including rats, mice, and humans. We use specialized culture media and supplements that are optimized for DRG neurons to promote cell growth and survival. Additionally, we use advanced techniques such as microfluidic chambers and micropatterning to create specialized environments that mimic the in vivo conditions of DRG neurons.

Creative Biolabs also offers a range of characterization services that enable the comprehensive analysis of DRG neurons. We use a combination of immunocytochemistry, electrophysiology, and gene expression analysis to identify and classify DRG neurons based on their sensory modality and neurotransmitter phenotype.

Fig.1 Primary culture of murine DRG neurons. (Asada, 2012)

Neurons Neurotoxicity Assay Capabilities

The DRG neurons neurotoxicity assay is a powerful tool for evaluating the potential neurotoxicity of drugs on sensory neurons. In this assay, isolated DRG neurons are cultured in vitro and exposed to various concentrations of test compounds. The viability of neurons is then assessed using a range of techniques, including fluorescent dyes, mitochondrial function assays, and high-content imaging. In addition to neuron survival, we also perform neurite outgrowth assays to examine the potential effects of exogenous compounds on neuronal activity and development, which providing valuable information on the potential of therapeutic compounds to either enhance or inhibit neurogenesis.

Fig.2 Neurite outgrowth and neurotoxicity of DRG neurons. (Unezaki, 2012)

Our Advantages

At Creative Biolabs, we are committed to helping our clients accelerate their drug development programs by providing innovative solutions and customized services. If you are looking for reliable and efficient DRG neuron characterization services, get in touch with us today to find out more about how we can support you in your endeavors!

References

1. Asada, Keiji, et al. "Age-related changes in afferent responses in sensory neurons to mechanical stimulation of osteoblasts in coculture system." American Journal of Physiology-Cell Physiology 302.5 (2012): C757-C765.
2. Unezaki, Sawako, et al. "Involvement of Tyr1472 phosphorylation of NMDA receptor NR2B subunit in postherpetic neuralgia in model mice." Molecular pain 8 (2012): 1744-8069.