Product
Human DBTRG-05MG Cells
[CAT#: NCB2108FY0101]
Human cells
- Species:
- Human
- Applications:
- 3D Cell Culture
- Cell Types:
- Glia
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.
Cell Types
Glia
Applications
3D Cell Culture
Relevant Diseases
Glioblastoma
Research Areas
Neural Cell
Marker
near tetraploid; range 87 to 91; most cells were missing copies of chromosome 10 and had extra copies of chromosome 7
Species
Human
Expression Profile
vimentin; S-100 protein; neuron specific enolase
Properties
Form
Frozen
Growth Pattern
Adherent
Morphology
Fibroblast
Culture Medium
The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
additional 30 mg/L L-proline
additional 35 mg/L L-cystine
additional 3.57 g/L HEPES
15 mg/L hypoxanthine
1 mg/L adenosine triphosphate
10 mg/L adenine
1 mg/L thymidine
fetal bovine serum to a final concentration of 10%
additional 30 mg/L L-proline
additional 35 mg/L L-cystine
additional 3.57 g/L HEPES
15 mg/L hypoxanthine
1 mg/L adenosine triphosphate
10 mg/L adenine
1 mg/L thymidine
Tissue Source
Brain
Age
59 years
Shipping
Dry ice
Storage
Vapor phase of liquid nitrogen
Handling Advice
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Research Use Only
For research use only
Donor Information
Gender
Female
Blood Type
Class I antigen expressed
Ethnicity
White
Additional Donor Information
The DBTRG-O5MG (Denver Brain Tumor Research Group 05) cell line was established from the tissue of a patient with glioblastoma multiforme who received local brain irradiation and multi-drug chemotherapy.
Publications
Publications (0)
Neural Cell Culture
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
