Human Brain Astroblastoma U-87 MG

[CAT#: NCL2110P117]

Species:: Human

Applications:: Cell Culture

Cell Types:: Tumor Cells

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Product Overview

Description

Human brain astroblastoma U-87 MG cell line was established by PontenJ et al., derived from malignant glioma. Nude mice can become tumors by subcutaneous inoculation.

Features

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00535.

Doubling time: 72 hours (PubMed=25984343); ~34 hours (PBCF).

Microsatellite instability: Stable (MSS) (Sanger).

Neurological Disease Models

Brain Tumor-related Cells

Cell Types

Tumor Cells

Applications

Cell Culture

Relevant Diseases

Astroblastoma

Species

Human

Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM High Glucose Medium + 10% Fetal Bovine Serum + 1% P/S

STR Analysis

Amelogenin X (AddexBio; CCRID; CLS; ECACC; PubMed=11416159; PubMed=22570425; PubMed=25877200; PubMed=27582061)

CSF1PO 10,11

D2S1338 20,23

D3S1358 16,17

D5S818 11,12

D7S820 8,9

D8S1179 10,11

D13S317 8,11

D16S539 12

D18S51 13 (CLS; PubMed=22570425)

13,14 (CCRID; PubMed=11416159; PubMed=25877200)

D19S433 15,15.2

D21S11 28,32.2

FGA 18,24

Penta D 9,14

Penta E 7,14

TH01 9.3

TPOX 8

vWA 15,17

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.