PP1 Binding Activity Assay (FP Assay)

Creative Biolabs has years of experience in the field of life science. With the development of neuroscience, the mechanisms of neurological diseases are constantly being solved by scientists and research institutes. The demand for research in neurological diseases has led us to establish a number of services to facilitate the development of therapeutics for neurological diseases more efficiently. Our professionals and technologies enable researchers to screen drug candidates or compounds to bind protein phosphatase 1 (PP1).

Background of Protein Phosphatase 1

Synaptic plasticity is involved in the efficacy of neuronal transmission, which contributes to the underlying biological mechanism of memory and learning. The process of synaptic plasticity is based on the mechanism of phosphorylation and dephosphorylation by protein kinases and protein phosphatases. The balance of phosphorylation and dephosphorylation processes is the key to maintaining synaptic plasticity, while imbalance of protein kinases and protein phosphatases may lead to neuropathological conditions. Therefore, the serine/threonine protein PP1 belonging to protein phosphatases plays an important role in synaptic plasticity and neuronal functions.

PP1 Binding Activity Assays

Creative Biolabs uses high-throughput fluorescence polarization (FP) assay to determine the PP1 binding activity of compounds. We use 384-well Low Dead Volume (LDV) microplates, which enable a large amount of compound screening. Fluorescein isothiocyanate (FITC)-labeled peptides and PP1 are then added for later incubation. FITC-labeled PP1 rotate rapidly in the solution and are excited by polarized light with FP signal. When the compounds bind with PP1 to form a complex, the emitted light becomes polarized as a result of the slow movement of the complex. The results of FP signal alterations can be read and calculated by our imaging and analytical platforms.

Fig.1 A basic setup for measuring fluorescence polarization in the laboratory. (Hall, 2016)

Applications

PP1 binding activity assay determined by FP assay can be used to screen compounds acting as PP1 inhibitors for maintaining the balance of protein kinases and protein phosphatases to treat neurological disorders. Our high-throughput technologies enable efficient screening of compounds.

Our Services

As a leading company in the neurological research field, Creative Biolabs is committed to providing comprehensive services to study synaptic plasticity and related indicators, such as PP1, and synaptic plasticity targeting therapies. Our services include but are not limited to in vivo, in vitro, and ex vivo services, discovery and development services for MoA studies, drug screening, and one-stop solutions to study neurodegenerative disorders, neuromuscular disorders, and other neurological disorders. Our teams offer corresponding cell lines and customized experimental protocol and support to meet your research requirement on synaptic plasticity. Please feel free to contact us for more detailed information on PP1 binding assay.

Reference

1. Hall, M. D.; et al. Fluorescence polarization assays in high-throughput screening and drug discovery: a review. Methods and applications in fluorescence. 2016, 4(2): 022001.