iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs)

Species:: Human

Cell Types:: Oligodendrocytes (OPC)

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Product Overview

Description

Oligodendrocytes belong to the family of glial cells in the human central and peripheral nervous system. They are multifunctional cells and are involved in many disabling neurological diseases, including adult-onset demyelinating diseases, leukocyte dystrophy and cerebral palsy.

Creative Biolabs' OPCs are induced oligodendrocyte progenitor cells that are reprogrammed from human fibroblasts using a proprietary reprogramming technology. OPCs cells were cryopreserved at a low passage number. OPCs cells are characterized by standard morphological examination and immunocytochemical methods for known marker proteins (such as O4, PDGFalphaR, NG2 and CNPase), and can be induced to express MBP under the induction of T3 hormone.

Cell Types

Oligodendrocytes (OPC)

Application Notes

Applications:

Disease modeling
Toxicity testing
Phenotypic Assays (e.g., expression of differentiation markers)
Research and Development of cell therapies in animal models
Studying oligodendrocyte differentiation, function, and myelination

Species

Human

Properties

Size

~1.05x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Quality Control

Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%).

Donor Information

Donor Health

Normal

Publications

Publications (0)

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Scientific Resources

[Brochure]

Neuros Celll Product Panel

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

I must say these cells are incredibly robust

They proliferate well and differentiate into mature oligodendrocytes efficiently. The cells arrived in perfect condition, and the support team was very responsive to my queries.

Excellent

I've been using these OPCs for my thesis research

The cells have excellent viability and exhibit expected marker expression during differentiation. The consistency in quality has made my work significantly smoother. Kudos to the company for such a reliable product.

Excellent

These OPCs are among the best we've used in our studies

As a PI, I am very particular about the reagents we use in our lab. The iNeu™ OPCs have exceeded my expectations. They differentiate beautifully into oligodendrocytes and are very responsive to various growth factors. This product has become a staple in our neurobiology projects.

Excellent

Great product overall

We have integrated OPCs into several of our ongoing projects. The cells have shown excellent performance in in vitro models of neurodegeneration. The comprehensive datasheet and user manual provided are very useful for setting up experiments.

Excellent

Excellent morphology and marker expression

Handling iNeu™ OPCs has been a breeze compared to other similar products. The cells exhibit high viability and consistent differentiation into oligodendrocytes. The packaging is also very secure, ensuring the cells arrive in optimal condition.

Q&As

Can you please recommend a medium?

The Oligodendrocyte Precursor Cell Growth Medium [CAT#: NCL-21P6-105] is used for culturing OPCs, and the Oligodendrocyte Precursor Cell Differentiation Medium [CAT#: NCL-21P6-106] is used for the OPC differentiation.

Should we seed the cells in a well of a 6-well plate or a T25 flask?

1) Seeding the iOligo cells (post-thaw) into 3 wells of a 6-well plate. Or, seed the iOligo cells into 1 single T25 flask. 2) Please do NOT over-passage the iOligo cells before differentiation. No more than 2X.

What’s the protocol for Matrigel coating (Corning)?

Please see Corning’ s instructions: dilute their Matrigel according to their lot# instructions. Typically, it is ~250µl- 400µl Matrigel in a 25ml solution (DMEM/F-12, for example). Then use 1ml to coat 1 well (6-well plate).

After thawing and centifuging in DMEM/F12. We resuspend in Oligodendrocyte Precursor Cell Growth Medium (no additional factors or Penstrep?)? And then plate in 3 wells of a 6-well plate? 1.5ml of media per well?

The cell culture media is serum-free and ready-to-use. Please do NOT dilute it further with anything! If you are using 6-well plates, each well gets ~1.5mL (instead of 1mL).

How many passages can the cells be cultured without compromising the phenotype of the cells?

We do not recommend more than 2X passages.

What´s the source of the cells?

Human fibroblasts cells.