iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs)
[CAT#: NCL-2103-P49]
- Species:
- Human
- Cell Types:
- Neural Progenitor Cell
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Oligodendrocytes belong to the family of glial cells in the human central and peripheral nervous system. They are multifunctional cells and are involved in many disabling neurological diseases, including adult-onset demyelinating diseases, leukocyte dystrophy and cerebral palsy.
Creative Biolabs' OPCs are induced oligodendrocyte progenitor cells that are reprogrammed from human fibroblasts using a proprietary reprogramming technology. OPCs cells were cryopreserved at a low passage number. OPCs cells are characterized by standard morphological examination and immunocytochemical methods for known marker proteins (such as O4, PDGFalphaR, NG2 and CNPase), and can be induced to express MBP under the induction of T3 hormone.
Creative Biolabs' OPCs are induced oligodendrocyte progenitor cells that are reprogrammed from human fibroblasts using a proprietary reprogramming technology. OPCs cells were cryopreserved at a low passage number. OPCs cells are characterized by standard morphological examination and immunocytochemical methods for known marker proteins (such as O4, PDGFalphaR, NG2 and CNPase), and can be induced to express MBP under the induction of T3 hormone.
Cell Types
Neural Progenitor Cell
Application Notes
Applications:
Disease modeling
Toxicity testing
Phenotypic Assays (e.g., expression of differentiation markers)
Research and Development of cell therapies in animal models
Studying oligodendrocyte differentiation, function, and myelination
Disease modeling
Toxicity testing
Phenotypic Assays (e.g., expression of differentiation markers)
Research and Development of cell therapies in animal models
Studying oligodendrocyte differentiation, function, and myelination
Species
Human
Properties
Size
~1.05x10^6 cells per 1ml of freezing medium (vial)
Growth Pattern
adherent
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%).
Neural Cell Lines
FQA
Can you please recommend a medium?
The Oligodendrocyte Precursor Cell Growth Medium [CAT#: NCL-21P6-105] is used for culturing OPCs, and the Oligodendrocyte Precursor Cell Differentiation Medium [CAT#: NCL-21P6-106] is used for the OPC differentiation.
Should we seed the cells in a well of a 6-well plate or a T25 flask?
1) Seeding the iOligo cells (post-thaw) into 3 wells of a 6-well plate. Or, seed the iOligo cells into 1 single T25 flask.
2) Please do NOT over-passage the iOligo cells before differentiation. No more than 2X.
What’s the protocol for Matrigel coating (Corning)?
Please see Corning’ s instructions: dilute their Matrigel according to their lot# instructions. Typically, it is ~250µl- 400µl Matrigel in a 25ml solution (DMEM/F-12, for example). Then use 1ml to coat 1 well (6-well plate).
After thawing and centifuging in DMEM/F12. We resuspend in Oligodendrocyte Precursor Cell Growth Medium (no additional factors or Penstrep?)? And then plate in 3 wells of a 6-well plate? 1.5ml of media per well?
The cell culture media is serum-free and ready-to-use. Please do NOT dilute it further with anything! If you are using 6-well plates, each well gets ~1.5mL (instead of 1mL).
How many passages can the cells be cultured without compromising the phenotype of the cells?
Cells are provided at passage 3. Upon thawing, cells are at passage 4. Cells should not be expanded beyond passage 6.
What´s the source of the cells?
Human fibroblasts cells.
For Research Use Only. Not For Clinical Use.
