iNeu™ Human Neural Stem Cell Line

[CAT#: NCL200552ZP]

A human neural stem cell line derived from human induced pluripotent stem cells.

Species:: Human

Cell Types:: Neural Stem Cell

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Product Overview

Description

Neural stem cells (NSC) are neural progenitor cells that can differentiate into different types of cells in the nervous system under certain conditions. Taking advantage of their continued reproduction, NSC has great potential for research in neurogenesis, neurodegenerative diseases, and clinical transplantation applications. NeuroShop's human neural stem cell line is derived from human-induced pluripotent stem cells, which are produced from adult skin fibroblasts using a unique, non-footprint free iPSC reprogramming method. When cultured in a medium containing bFGF and EGF, these cells can normally proliferate without differentiation into neuronal cells.

Main features
Derived from human iPS cells without footprints
Homogeneous population
Under certain conditions, it can differentiate into more than 90% of neurons

Cell Types

Neural Stem Cell

Species

Human

Properties

Size

1x10e6 cells/vial

Form

Frozen Cryovials/Live Culture

Tissue Source

Human iPSC

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Cells are negative for mycoplasma contamination.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

After recovery from cryopreservation, each batch of hNSC is tested for growth and viability. In addition, each batch was tested for Nestin expression to ensure its undifferentiated properties.