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Creative Biolabs

HOT iNeu™ Human Neural Stem Cell Line

[CAT#: NCL200552ZP] Review(5) Q&As(3)

A human neural stem cell line derived from human induced pluripotent stem cells.

Species:
Human
Cell Types:
Neural Stem Cells

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Product Overview

Description

Neural stem cells (NSC) are neural progenitor cells that can differentiate into different types of cells in the nervous system under certain conditions. Taking advantage of their continued reproduction, NSC has great potential for research in neurogenesis, neurodegenerative diseases, and clinical transplantation applications. NeuroShop's human neural stem cell line is derived from human-induced pluripotent stem cells, which are produced from adult skin fibroblasts using a unique, non-footprint free iPSC reprogramming method. When cultured in a medium containing bFGF and EGF, these cells can normally proliferate without differentiation into neuronal cells.

Main features
Derived from human iPS cells without footprints
Homogeneous population
Under certain conditions, it can differentiate into more than 90% of neurons

Cell Types

Neural Stem Cells

Species

Human
Properties

Size

1x10e6 cells/vial

Form

Frozen Cryovials/Live Culture

Tissue Source

Human iPSC

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Cells are negative for mycoplasma contamination.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

After recovery from cryopreservation, each batch of hNSC is tested for growth and viability. In addition, each batch was tested for Nestin expression to ensure its undifferentiated properties.
Donor Information

Donor Health

Normal
Publications

Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
The cells are robust and consistent
They differentiate well into neurons and glial cells, and the viability after thawing is impressive. Customer support has been very responsive to any queries.
user
Excellent
We've appreciated the technical support available from the company
iNeu™ human neural stem cells have been a game-changer for our experiments. The differentiation protocols provided are clear and effective, leading to high purity neural cultures. The cells show expected phenotypic markers, and we've had reproducible results across multiple batches.
user
Excellent
A reliable tool for our studies on neural differentiation
As a technician, I appreciate the ease of handling and robustness of the iNeu™ stem cells. They recover well from freezing, and we’ve observed minimal batch-to-batch variability. The accompanying documentation is comprehensive, making it easier to follow the differentiation protocols.
user
Excellent
A robust platform for screening
We use cells to screen neuroactive compounds. The cells have proven to be a reliable model, exhibiting appropriate responses to our test compounds. Their consistent quality and phenotype make them ideal for high-throughput screening.
user
Excellent
This product has significantly streamlined my workflow
The cells have been an excellent resource for my research. The cells are easy to work with and differentiate reliably. This has allowed me to focus on my experimental design rather than troubleshooting cell culture issues.
Q&As
What's the related media?
The related media is Human Neural Stem Cell Growth Medium [CAT#: NCL-2105-P140-IX]
How stable are neural stem cell line cultures over long-term passages, and are there any specific considerations for passaging these cells?
Cells are stable over multiple passages when maintained under appropriate conditions. We advise regular monitoring of cell morphology and growth characteristics to identify optimal passaging intervals. Detailed instructions on passaging techniques are included to maximize cell viability and functionality.
Can cell line be customized or modified to suit specific research needs or experimental conditions?
Yes, we offer customization options to tailor cells according to specific research requirements, such as genetic modifications or specialized culture conditions. Contact our technical team to discuss customization options and feasibility for your study.
For Research Use Only. Not For Clinical Use.
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