Rat C6 Cells

[CAT#: NCB2108FY0068]

Animal cells

Species:: Rat

Applications:: 3D Cell Culture

Cell Types:: Glia

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Request Pricing

Inquiry

Product Overview

Description

Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Cell Types

Glia

Applications

3D Cell Culture

Application Notes

This cell line is a suitable transfection host.

Relevant Diseases

Glioma

Research Areas

Neural Cell

Marker

Stemline number is diploid. Karyotype is stable within the stemline number and is that of a normal male. Three cells with breaks; one with a secondary constriction, one with a dicentric, one with a rearrangement and four with terminal or centromere associations.

Species

Rat

Expression Profile

S-100 protein; glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin

Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Fibroblast

Culture Medium

The base medium for this cell line is F-12K Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.

Freeze Medium

Complete growth medium supplemented with 5% (v/v) DMSO

Tissue Source

Brain

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Research Use Only

For research use only

Donor Information

Additional Donor Information

Benda et al. cloned the glial cell line C6 from rat glioma induced by N-nitrosomethylurea. After a series of alternating cultural and animal passages.

Publications