Rat Stable PC12 Cell Line 150Q, Huntington's Disease Model

[CAT#: NCL-7P065]

Species:: Rat

Applications:: In Vivo Like Assay

Cell Types:: iPSC

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Product Overview

Description

Huntington's disease (HD) is an autosomal dominant degenerative disease caused by the amplification of polyglutamine (>37 units) in the Huntington gene. Huntingtin 150Q Stable PC12 cell line is a rat pheochromocytoma cell line that expresses the N-terminal fragment of Huntingtin protein containing 150 glutamine residues. HD 150Q protein is mainly found in the nucleus, so these cells lack normal neurite development. In contrast, its control cell line Huntingtin 20Q Stable PC12 cell line displays HD 20Q protein evenly in the cytoplasm. Together, these two cell lines provide a valuable model for studies related to changes in gene expression caused by polyglutamine tracts and HD pathology.

Details:
Tissue: Renal
Growth Properties: Adherent
Cell Morphology: Small|Irregular
Seeding Density: 30,000 - 50,000 cells/cm2
Population Doubling Time: 40 - 50 hours

Cell Types

iPSC

Applications

In Vivo Like Assay

Application Notes

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. NCL-2105-P169-AM. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (NCL-2105-P170-AM)* to a final concentration of 5%, horse serum to the final concentration of 10% and Penicillin/Streptomycin Solution (NCL-2105-P172-AM) to a final concentration of 1%.