Human iPSC Line (Episomal, HFF-derived)
[CAT#: NCL-2103-P87]
- Species:
- Human
- Applications:
- Cell Assay
- Cell Types:
- iPSC
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Footprint-free human iPS (induced pluripotent stem) cell line was derived from human foreskin fibroblasts (HFFs) by ectopic expression of OCT4, SOX2, KLF4, and L-MYC genes using Creative Biolabs episomal plasmids. Use morphological selection criteria to derive cells without the use of fluorescent markers or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is the same as that of human ES cells. The cells express pluripotency markers OCT4, SSEA-3, Nanog and endogenous alkaline phosphatase. High viability, low passage iPS cells have been pre-adapted to serum-free and feeder-free culture conditions.
Cell Types
iPSC
Applications
Cell Assay
Species
Human
Properties
Size
5x10^5 cells/vial
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry Ice - Overnight Shipping
Storage
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 12 months when stored as directed.
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Human iPS cells were grown in mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of OCT4 and TRA-1-60, as well as the activity of alkaline phosphatase.
For Research Use Only. Not For Clinical Use.
