Creative Biolabs

Embryonic Fibroblasts (MEFs)-Derived iPS Cell Line (Mouse)

[CAT#: NCL200551ZP]

A mouse iPS (induced pluripotent stem) cell line derived from mouse embryonic fibroblasts (MEFs) by retroviral expression of Oct3/4, Sox2, Klf4 and c-Myc genes.

Species:
Mouse
Applications:
Cell Assay
Cell Types:
iPSC

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Description
The mouse iPS cell line (induced pluripotent stem cell line) is derived from mouse embryonic fibroblasts (MEF) through retroviral expression of Oct3/4, Sox2, Klf4, and c-Myc genes. Morphological selection criteria are used to derive cells. When cultured under standard mouse ES cell culture conditions, the morphology of mouse iPSC is the same as that of mouse ES cells. These cells also expressed the pluripotency markers SSEA-1 and Nanog, and showed strong endogenous alkaline phosphatase activity. Mouse iPS cells grow on the feeder layer of mouse embryonic fibroblasts (MEF), and the plates need to be pretreated with gelatin.
Cell Types
iPSC
Applications
Cell Assay
Relevant Diseases
Neurological Disorders
Marker
SSEA-4/ Nanog
Species
Mouse
Size
2x10e5 cells/vial
Form
Frozen Cryovials/Live Culture
Cell State
Live cells are in mid log-phase growth.
Tissue Source
Human foreskin fibroblasts (HFFs)
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
Cells are negative for mycoplasma contamination.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control
Mouse iPS cells were grown in mouse ES medium supplemented with 103 U/ml LIF. After recovery from cryopreservation, each batch of mouse iPS cells was tested for growth and viability. In addition, the SSEA-1 and Nanog expression and alkaline phosphatase activity of each batch of cell lines were also tested.

For Research Use Only. Not For Clinical Use.
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