Creative Biolabs

Mesenchymal Stromal Cell (MSC)-Derived iPS Cell Line, Episomal (Human)

[CAT#: NCL200544ZP]

A footprint-free human iPS (induced pluripotent stem) cell line was derived from human mesenchymal stromal cells (MSCs) by ectopic expression of OCT4, SOX2, KLF4, and L-MYC genes using episomal plasmids.

Species:
Human
Applications:
Cell Assay
Cell Types:
iPSC

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Description
The cells were derived using morphological selection criteria. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is the same as human ES cells. These cells also expressed pluripotency markers TRA-1-60, SSEA-3 and Oct4, and showed strong endogenous alkaline phosphatase activity.
Cell Types
iPSC
Applications
Cell Assay
Relevant Diseases
Neurological Disorders
Marker
TRA-1-60/ SSEA-3/ Oct4
Species
Human
Size
2x10e5 cells/vial
Form
Frozen Cryovials/Live Culture
Cell State
Live cells are in mid log-phase growth.
Tissue Source
Human mesenchymal stromal cells (MSCs)
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
Cells are negative for mycoplasma contamination.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control
Human iPS cells were cultured in mTeSR1 medium without feeder layers. After recovery from cryopreservation, each batch of human iPS cells was tested for growth and viability. In addition, each batch was tested for TRA-1-60 and Oct4 expression and alkaline phosphatase activity.

For Research Use Only. Not For Clinical Use.
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