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Creative Biolabs
Product

Human iPS Cell Line (Episomal, CD34+, ApoE2)

[CAT#: NCL-2103-P88]

Species:
Human
Cell Types:
iPSC

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Product Overview

Description

Through the use of sophisticated genome editing technologies, the Creative Biolabs footprint-free human iPS (induced pluripotent stem) cell line bearing the APOE-2 mutation was created from the syngeneic iPSC line (catalog number NCL-2103-P91) of the company. As a consequence of stable homozygous transformation using Cys112 (TGC) from Arg112 (CGC) and Cys158 (TGC) from Arg158 (CGC) in the APOE gene, the results demonstrated that iPS46 is a homozygous line.

Neurological Disease Models

AD-related Cells

Cell Types

iPSC

Relevant Diseases

Alzheimer's Disease

Species

Human
Properties

Size

5x10^5 cells/vial

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry Ice - Overnight Shipping

Storage

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4, as well as the activity of alkaline phosphatase.
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For Research Use Only. Not For Clinical Use.
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