Human iPS Cell Line (Retroviral)

[CAT#: NCL-2103-P84]

Species:: Human

Cell Types:: iPSC

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Product Overview

Description

Human foreskin fibroblasts (HFF) are used to create the human iPS (induced pluripotent stem) cell line through retroviral expression of the OCT4, SOX2, KLF4, and c-MYC genes. To generate cells without the use of fluorescent markers or drug selection, employ morphological selection criteria. Human iPS cells have the same morphology as human ES cells when grown under the same circumstances as regular human ES cell culture. These cells exhibit strong endogenous alkaline phosphatase and the pluripotency markers SSEA-4 and Nanog.

Cell Types

iPSC

Species

Human

Properties

Size

5x10^5 cells/vial

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry Ice - Overnight Shipping

Storage

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Human iPS cells were grown in human ES medium supplemented with 10 ng/ml of bFGF. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of SSEA-4 and Nanog, as well as the activity of alkaline phosphatase.

Donor Information