Human iPSC Line (Episomal, Cord Blood-derived)
[CAT#: NCL-2103-P82]
- Species:
- Human
- Cell Types:
- iPSC
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Using episomal plasmids, the OCT4, SOX2, KLF4, L-MYC, and Lin28 genes were ectopically expressed in human new-born cord blood (CB) mononuclear cells to create a footprint-free human iPS (induced pluripotent stem) cell line. To generate cells without the use of fluorescent markers or drug selection, employ morphological selection criteria. Human iPS cells have the same morphology as human ES cells when grown under the same circumstances as regular human ES cell culture. These cells additionally exhibit significant endogenous alkaline phosphatase activity and the pluripotency markers TRA-1-60, SSEA-3, and Oct4. IPS cells with high viability and low passage have already acclimated to culture conditions without serum and feeders.
Cell Types
iPSC
Species
Human
Properties
Size
>5x10^5 cells/vial
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry Ice - Overnight Shipping
Storage
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 12 months when stored as directed.
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4, as well as the activity of alkaline phosphatase.
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
