Human iPSC Line (Episomal, Cord Blood-derived)
[CAT#: NCL-2103-P82]
- Species:
- Human
- Applications:
- Cell Assay
- Cell Types:
- iPSC
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Footprint-free human iPS (induced pluripotent stem) cell line was derived from human new born cord blood (CB) mononuclear cells by ectopic expression of OCT4, SOX2, KLF4, L-MYC and Lin28 genes using episomal plasmids. Use morphological selection criteria to derive cells without the use of fluorescent markers or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is the same as that of human ES cells. These cells also express pluripotency markers TRA-1-60, SSEA-3 and Oct4, and show strong endogenous alkaline phosphatase activity. High viability, low passage iPS cells have been pre-adapted to serum-free and feeder-free culture conditions.
Cell Types
iPSC
Applications
Cell Assay
Species
Human
Size
>5x10^5 cells/vial
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry Ice - Overnight Shipping
Storage
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 12 months when stored as directed.
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and Oct4, as well as the activity of alkaline phosphatase.
For Research Use Only. Not For Clinical Use.
