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NeuroMab™ Anti-SEZ6 Antibody, Clone NR24P

[CAT#: NRP-0422-P511] Review(5) Q&As(3)

A functional antibody raised against Human, Mouse, Rat SEZ6.

Host Species:
Mouse
Species Reactivity:
Human; Mouse; Rat
Applications:
FC; IHC; In Vitro; In Vivo

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Product Overview

Description

The antibody specifically binds to a human SEZ6 protein, use for the treatment, diagnosis or prophylaxis of neoplastic disorders.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Clone Number

NR24P

Applications

FC; IHC; In Vitro; In Vivo

Relevant Diseases

Epilepsy
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

SEZ6

Official Name

SEZ6

Full Name

Seizure Related 6 Homolog

Alternative Names

Seizure Related 6 Homolog; Seizure Related 6 Homolog (Mouse); Seizure Protein 6 Homolog; HSEZ-6; BSRPC; SEZ-6;

Gene ID

20370(Mouse); 192247(Rat)

Uniprot ID

Q7TSK2(Mouse)
Product Pictures
FuncS

Figure 1 shows the detection of SEZ6 protein expression in NTX tumor cells by flow cytometry using various anti-SEZ6 antibodies.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

FuncS

Figure 2 shows the results of an in vitro disruption assay using anti-SEZ6 ADCs in HEK293 cells overexpressing SEZ6.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

FuncS

Figure 3 shows the effect of anti-SEZ6 ADCs on the growth of SCLC (LU86) and LCNEC (Lu50) tumors in vivo.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

FuncS

Figure 4 shows the ability of conjugated humanized anti-SEZ6 antibodies to delay the growth of four SCLC tumors (LU80, LU64, LU111 and LU117) in vivo and achieve durable remission in immunosuppressed mice.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

FuncS

Figure 5 shows the ability of conjugated humanized anti-SEZ6 antibodies to delay the growth of four SCLC tumors (LU80, LU64, LU111 and LU117) in vivo and achieve durable remission in immunosuppressed mice.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
Performed exceptionally well in our in vivo studies
We needed an antibody that could specifically target SEZ6 in live tissue, and this one did not disappoint. The staining was robust and specific, allowing us to track SEZ6 expression in real-time effectively.
user
Excellent
This antibody helped us identify significant findings in our SEZ6-related research
I conducted a series of in vitro experiments using the antibody and was very pleased with the results. The antibody showed high affinity and specificity for SEZ6, and we observed minimal background staining.
user
Excellent
This antibody worked wonderfully
The staining was very specific, with no cross-reactivity, and the signal was strong even at low concentrations. We used it to study SEZ6 expression in various brain regions, and the results were clear and reproducible.
user
Excellent
We used the antibody in our flow cytometry experiments and found it to be highly specific and reliable
The antibody provided consistent results across multiple samples and experiments. The clear distinction between SEZ6-positive and SEZ6-negative cells was impressive, making our data analysis straightforward.
user
Excellent
It was easy to use and produced reproducible results
It demonstrated high specificity and affinity for SEZ6, allowing us to achieve clear, consistent results in our assays. This antibody has become a crucial tool in our research on SEZ6 function and expression.
Q&As
How does this antibody perform in detecting SEZ6 protein in brain tissue sections?
The antibody performs exceptionally well in detecting SEZ6 protein in brain tissue sections. It provides high specificity and sensitivity, allowing for clear visualization of SEZ6 localization within neural tissues. The antibody's robust performance in immunohistochemistry makes it ideal for studying SEZ6 expression patterns and distribution in various brain regions.
Can you provide any tips for troubleshooting if the antibody does not perform as expected?
If you encounter issues with the antibody, we recommend the following troubleshooting tips: Verify the antibody’s storage conditions and expiration date. Ensure proper sample preparation and handling, including fixation methods for tissue sections. Optimize antibody dilution and incubation times. Use appropriate controls to confirm specificity. If problems persist, our technical support team is available to assist with detailed guidance and troubleshooting protocols to help resolve any issues.
What is the typical lead time for ordering this antibody?
The typical lead time for ordering the antibody is 1-2 weeks, depending on current stock availability. We strive to maintain adequate inventory to fulfill orders promptly. For large quantities or special requests, lead times may vary, and we recommend contacting our customer service team to obtain accurate delivery estimates and ensure timely receipt of your antibody.
For Research Use Only. Not For Clinical Use.
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