pH Indicators

Numerous physiological functions, such as the contraction of muscles, the transmission of nerve impulses, and cell signaling, are heavily dependent on variations in intracellular pH. The shift in intracellular pH might occur gradually, with variations occurring only in fractions of a pH unit. These days, Creative Biolabs provides a variety of innovative indicators that let you keep an eye on the pH in the cytoplasm, particularly in organelles.

pH indicators

pH indicators at Creative Biolabs

Functional Gene	Description
pHTomato	pHTomato is a bright, red, pH-sensitive, genetically encoded probe. This probe can be used to construct indicators that allow imaging of neurotransmitter release to monitor neuronal activity and the tight size match between pre- and postsynaptic compartments, as well as the intriguing target cell-dependent regulation of presynaptic vesicles observed in cisternae.
pHoran4	pHoran4 is a subset of the pH-sensitive mOrange proteins characterized by the M163K mutation near the chromophore. This probe demonstrates the key role of this residue in regulating the pH sensitivity of discoid red fluorescent protein variants.
pHmScarlet	pHmScarlet is a red fluorescent protein probe with excellent brightness, stability, and pH sensitivity. This probe has the highest pH sensitivity of any existing red fluorescent protein, can detect more secretion events, and can identify both the vesicle anchoring and fusing processes of secretion events.
pHuji	pHuj is derived from the red fluorescent protein mApple K163Y, a variant that exhibits fluorescence intensity by more than 20-fold between pH 5.5 and 7.5. pHuji is ideal for the detection of individual exocytosis and endocytosis events.
pHRed	pHRed is a red fluorescent protein (RFP) that combines with the pH sensitivity of the A213S mutant. pHRed can detect intracellular pH fluctuations by imaging energy-dependent changes in cytosolic and mitochondrial pH.

We have the equipment, experience, and procedural flexibility to provide custom AAV tools that are tailored to your or your organization's specific requirements. If you would like more information about any products or services you are interested in, don't hesitate to get in touch with us.

Intellectual Property

Creative Biolabs is a contract manufacturer that produces tailored viruses and vectors according to client specifications. Importantly, this manufacturing service does not grant Creative Biolabs any intellectual property ownership; all designs and associated intellectual property rights remain exclusively with the customer.

Certain vector components may be subject to third-party intellectual property rights. Creative Biolabs offers publicly available sequence information for these components as a convenience for reference only. The customer assumes full responsibility and liability for the use of these components in their custom vectors. By purchasing vectors containing such components, the customer confirms they have secured all necessary intellectual property licenses, including the right to engage Creative Biolabs for manufacturing.

For Research Use Only. Not For Clinical Use.

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