Human Microglia Cell Line HMC3, Immortalized

Species:: Human

Applications:: Cell Culture

Cell Types:: Microglia

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Product Overview

Cell Types

Microglia

Applications

Cell Culture

Species

Human

Properties

Size

>1x10^6 Cells

Growth Pattern

Adherent

Morphology

Macrophage-like

Culture Medium

MEM + 10% FBS + 1% P/S

Freeze Medium

55% Culture Media+40%FBS+5%DMSO

Tissue Source

Brain

Shipping

Dry ice.

Storage

Liquid Nitrogen

Handling Advice

Passage ratio:1:3-1:8
Changing cell medium: 2~3 times/week

Research Use Only

For research use only

Quality Control

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The cells showed robust proliferation and maintained typical microglial morphology

Impressed with the HMC3 cell line's ability to mimic primary microglia behavior, essential for our investigations into Alzheimer's disease pathogenesis. The cells exhibited significant phagocytic activity and cytokine secretion patterns akin to activated microglia.

Excellent

Excellent compatibility

The HMC3 cells demonstrated excellent compatibility with our imaging assays, allowing detailed visualization of microglial dynamics in response to various stimuli. Their consistent performance enabled high-content screening of anti-inflammatory compounds.

Excellent

Stable phenotype

We chose the HMC3 cell line for its well-documented transcriptomic profile resembling primary microglia. This authenticity facilitated accurate interpretation of RNA-seq data, aiding our studies on gene expression changes in neuroinflammatory conditions.

Excellent

Great authenticity and suitability

The HMC3 cells' expression of key microglial markers validated their authenticity and suitability for immunocytochemical assays. Their consistent antigen expression patterns facilitated accurate characterization of microglial activation states.

Excellent

Leveraged cell line's phagocytic capacity

We leveraged the HMC3 cell line's phagocytic capacity to study its implications in neurodegenerative clearance mechanisms. The cells' efficient engulfment of fluorescent particles enabled quantitative analysis of phagocytic activity.

Q&As

What are the key features that distinguish the HMC3 cell line from other microglia cell lines available on the market?

The HMC3 cell line is derived from human microglia and is immortalized, ensuring stable growth and reproducibility in experiments. It retains crucial microglial characteristics such as phagocytic activity and cytokine secretion profiles, making it ideal for studying neuroinflammation and neurodegenerative diseases.

What culture conditions are recommended for optimal growth and maintenance of the HMC3 cell line?

The HMC3 cell line thrives under standard cell culture conditions with DMEM/F-12 medium supplemented with FBS and antibiotics. Regular passage and careful monitoring of cell health are essential to maintain optimal growth and functionality, ensuring the cells perform consistently in experimental settings.

What are the advantages of using an immortalized cell line like HMC3 compared to primary microglia isolation for research purposes?

The HMC3 cell line offers advantages such as unlimited supply, consistent phenotype, and reduced variability compared to primary microglia isolation. It simplifies experimental logistics, allowing researchers to focus on specific research questions without concerns about donor variability or limited cell availability.