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iNeu™ Human Astrocyte
- Species:
- Human
- Cell Types:
- Astrocytes
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Astrocyte dysfunction has been implicated in a number of neurological conditions such as Alzheimer's and Parkinson's diseases, autism, amyotrophic lateral sclerosis (ALS), Rett syndrome and schizophrenia.
Creative Biolabs's Human iPSC-derived astrocytes offer a convenient and physiologically-relevant tool to study these cells as an isolated population or within co-cultures of other neural cells for analysis of complex central nervous system (CNS) circuitry.
Creative Biolabs's Human iPSC-derived astrocytes express glial markers such as glial fibrillary acidic protein (GFAP), S100b, AQP4, ALDHL1L and EAAT1.
They have also been further validated for expression of other relevant markers including Vimentin, Kir4.1 (potassium channel) and CD44.
These cells exhibit low expression of Nestin, a marker of neural stem cells, and are tested to have very low or negative expression for MAP2, a marker of neurons.
Creative Biolabs's Human iPSC-derived astrocytes offer a convenient and physiologically-relevant tool to study these cells as an isolated population or within co-cultures of other neural cells for analysis of complex central nervous system (CNS) circuitry.
Creative Biolabs's Human iPSC-derived astrocytes express glial markers such as glial fibrillary acidic protein (GFAP), S100b, AQP4, ALDHL1L and EAAT1.
They have also been further validated for expression of other relevant markers including Vimentin, Kir4.1 (potassium channel) and CD44.
These cells exhibit low expression of Nestin, a marker of neural stem cells, and are tested to have very low or negative expression for MAP2, a marker of neurons.
Cell Types
Astrocytes
Species
Human
Properties
Growth Pattern
Adherent
Biosafety Level
Level 1
Tissue Source
Cord blood CD34+ cells
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
Donor Information
Donor Health
Normal
Publications
Publications (0)
Neural Cell Lines
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentHighly recommended for anyone working in neurobiologyWe are studying neuroinflammatory responses, and these cells have shown consistent and reproducible results across multiple experiments. The cells arrive in excellent condition, and their viability is impressive. We also appreciate the detailed protocol provided, which has helped streamline our workflow.
ExcellentWe have used the astrocytes in our research on astrocyte-neuron interactionsThe cells integrate well into our co-culture systems, maintaining their characteristic morphology and functionality. The customer support team has been very responsive and helpful, addressing any queries we had.
ExcellentExtremely satisfied with their performanceThey exhibit robust growth and respond well to various treatments in our assays. The purity and quality of the cells are top-notch, and the shipping process ensures they arrive in perfect condition. We have seen consistent results, making our data highly reliable.
ExcellentThe results have been outstandingTheir response to stimuli in our studies on CNS disorders is highly consistent, providing reliable data. The technical support provided by the company has been exceptional, guiding us through initial setup and troubleshooting.
ExcellentContributing to more accurate and reproducible experimentsWe incorporated the astrocytes into our research on brain tumors, and the results have been outstanding. The cells maintain their phenotype and show high viability. The comprehensive documentation and protocols provided with the product are extremely useful.
Q&As
Do you have different types of human astrocytes available?
There are two subtypes of astrocytes: Type I and Type II. >90% of our astrocytes are Type II.
Can astrocytes be co-cultured with other cell types for studying cellular interactions?
Yes, human astrocytes can be co-cultured with various cell types, such as neurons, microglia, or endothelial cells, to study cellular interactions and communication. Co-culturing allows researchers to investigate how astrocytes influence or are influenced by other cells within the brain environment, providing a more comprehensive understanding of cellular networks and signaling pathways.
How should human astrocytes be stored and handled to maintain their integrity?
Human astrocytes should be stored in liquid nitrogen if not used immediately to preserve their viability and functionality. Upon thawing, they should be handled carefully, avoiding prolonged exposure to ambient temperatures. It is essential to follow recommended protocols for thawing and plating to minimize stress and ensure optimal cell health.
For Research Use Only. Not For Clinical Use.
