Calcium Sensors

In the mammalian nervous system, calcium is an important intracellular signaling molecule in neurons. When neurons are active, the intracellular calcium ion concentration can increase 10-100 times. Calcium indicators that are genetically encoded (GECI) are widely employed. GECI is a fluorescent protein that binds to a calcium-binding protein like calmodulin or troponin C. The top-selling genetically encoded calcium indicators are currently GCaMP, Pericams, Cameleons, TN-XXL, and Twitch. GCaMP6 is particularly useful for in vivo calcium imaging research due to its high sensitivity.

GECIs
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GECIs

Variant	Description
GCaMP6	There are three distinct subtypes of the sixth generation of GCaMP protein (GCaMP6), each with unique properties: GCaMP6s, GCaMP6m, and GCaMP6f, each with different characteristics. GCaMP6s is highly sensitive and suitable for the indication of low-frequency signals. GCaMP6f has a fast kinetic curve and the fastest dissociation, which is ideal for the indication of high-frequency signals. You need to choose according to your experimental needs.
jGCaMP7	The jGCaMP7 family contains four different types of proteins: jGCaMP7s has high sensitivity and is suitable for the detection of low-frequency signals; jGCaMP7f has fast kinetics and is suitable for the stronger detection of single action potential responses. jGCaMP7b has a higher background fluorescence, making it useful for identifying neural processes or nerve fibers; jGCaMP7c has the lowest background fluorescence, with a clear signal and good contrast, making it ideal for range imaging.
XCaMP	Based on CaMKK, XCaMP is produced by mutation screening and is available in four color variants: blue (XCaMP-B), green (XCaMP-G), orange (XCaMP-O), and red (XCaMP-R). XCaMP-G has a greater fluorescence intensity than GCaMP6, and it also performs noticeably better than GCaMP6 in the calcium ion response to action potential stimulation. When XCaMP is used in a neuron-specific expression approach, the activity of three different types of neurons in a certain behavior can be monitored concurrently in a freely moving state. This XCaMP, when used in two-photon microscopy for microstructural functional imaging, allows for simultaneous two-color imaging of presynaptic and postsynaptic structures.
CaMPARI	CaMPARI is a new calcium imaging method that considers both global and microscopic characteristics. CaMPARI can convert instantaneous neuronal activity into permanent red fluorescent protein expression, including CaMPARI and CaMPARI2.

Creative Biolabs provides extensive custom GECI services. Please contact us for details. We also offer related AAV packaging services and readily available AAV kits.

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Creative Biolabs is a contract manufacturer that produces tailored viruses and vectors according to client specifications. Importantly, this manufacturing service does not grant Creative Biolabs any intellectual property ownership; all designs and associated intellectual property rights remain exclusively with the customer.

Certain vector components may be subject to third-party intellectual property rights. Creative Biolabs offers publicly available sequence information for these components as a convenience for reference only. The customer assumes full responsibility and liability for the use of these components in their custom vectors. By purchasing vectors containing such components, the customer confirms they have secured all necessary intellectual property licenses, including the right to engage Creative Biolabs for manufacturing.

For Research Use Only. Not For Clinical Use.

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